Label-free identification of trace microcystin-LR with surface-enhanced Raman scattering spectra

doi:10.1016/j.talanta.2018.11.072

	Label-free identification of trace microcystin-LR with surface-enhanced Raman scattering spectra
	He, Shixuan1,2 ; Xie, Wanyi1 ; Fang, Shaoxi1 ; Zhou, Daming1 ; Djebbi, Khouloud 1; Zhang, Zhiyou 2; Du, Jinglei 2; Du, Chunlei1,2 ; Wang, Deqiang1
刊名	TALANTA
	2019-04-01
卷号	195 页码:401-406
关键词	Microcystin-LR Surface-enhanced Raman scattering Principal component analysis
ISSN号	0039-9140
DOI	10.1016/j.talanta.2018.11.072
通讯作者	He, Shixuan(heshixuan@cigit.ac.cn) ; Du, Chunlei(cldu@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
英文摘要	The analysis of trace microcystin-LR (MC-LR) plays important roles in environmental fields, especially in monitoring domestic water quality and safety, since it has particularly harmful effect on wild and domestic animals as well as humans at low doses. Herein, we combine confocal Raman spectroscopy with SERS-AG substrate to characterize the "fingerprint" information of MC-LR directly. High sensitivity of SERS-AG substrates was verified by utilizing the probe molecule Rhodamine 6 G. Mapping spectra demonstrated good reproducibility of MC-LR identification with label-free surface-enhanced Raman scattering (SERS) strategy. Differences between SERS spectra of MC-LR and R6G, microcystin-RR were evaluated by calculating their scores and loading weights with an unsupervised exploratory principal component analysis method. Then, relationship between Raman intensities and concentrations was preliminary analyzed with SERS spectra of MC-LR and the lowest concentration of MC-LR identification was 10(-6) mg L-1 while using SERS-AG substrate. Thereafter, 68.6% quantitative recovery of 10(-3) mg L-1 MC-LR in tap water samples was obtained by the proposed label-free SEAS method. These results showed that confocal Raman spectroscopy with label-free surface-enhanced Raman scattering strategy can handle the identification of trace MC-LR for monitoring water quality and safety worldwide in future.
资助项目	Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0310] ; Chongqing Science and Technology Innovation in Social Livelihood of the People[cstc2015shmszxX0002] ; National Natural Science Foundation of China[21507131] ; National Natural Science Foundation of China[41603089]
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000456762000052
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/7397]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	He, Shixuan; Du, Chunlei; Wang, Deqiang
作者单位	1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, ShuiTu Technol Dev Zone, 266 Fangzheng Ave, Chongqing 400714, Peoples R China 2.Sichuan Univ, Dept Phys, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	He, Shixuan,Xie, Wanyi,Fang, Shaoxi,et al. Label-free identification of trace microcystin-LR with surface-enhanced Raman scattering spectra[J]. TALANTA,2019,195:401-406.
APA	He, Shixuan.,Xie, Wanyi.,Fang, Shaoxi.,Zhou, Daming.,Djebbi, Khouloud.,...&Wang, Deqiang.(2019).Label-free identification of trace microcystin-LR with surface-enhanced Raman scattering spectra.TALANTA,195,401-406.
MLA	He, Shixuan,et al."Label-free identification of trace microcystin-LR with surface-enhanced Raman scattering spectra".TALANTA 195(2019):401-406.