Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro

doi:10.1016/j.colsurfa.2019.04.007

	Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro
	Wang, Di 1,2; Wang, Meiyue 2,3; Wang, Anhe 2; Li, Jieling 2; Li, Xin 2,3; Jian, Honglei 2; Bai, Shuo 2; Yin, Jian 1
刊名	COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
	2019-07-05
卷号	572 页码:266-273
关键词	Collagen Microspheres Emulsification Macrophage 3D proliferation
ISSN号	0927-7757
DOI	10.1016/j.colsurfa.2019.04.007
英文摘要	Macrophages, as an important member of immune cells, have now become a research hotspot in the field of immunotherapy, which requires prominent proliferation in vitro for clinical use. Herein, we reported the fabrication of collagen/chitosan microspheres via emulsification for 3D macrophages proliferation. With glutaraldehyde (GA) as cross-linker, the microspheres have an average diameter of 200 mu m and positively charged surfaces, which are of great stability and biocompatibility. Compared with 2D cell proliferation, the 3D microspheres could dramatically enhance macrophages proliferation rate and promote their biological functions. Furthermore, macrophages on the surface of microspheres could be easily collected by blowing and suction, without the need of trypsin digesting or the degradation of microsphere. Hence the microspheres could be reused for macrophage proliferation repeatedly. This study highlights opportunities for development of high-effective cell proliferation approach and provides a great promise in immune cell based immunotherapy.
资助项目	National Natural Science Foundation of China[21703253] ; National Natural Science Foundation of China[21774132] ; National Natural Science Foundation of China[21877052] ; National Natural Science Foundation of China[31700706] ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province[BK20180030] ; Fundamental Research Funds for the Central Universities[JUSRP51712B] ; Max Planck Society International Partner Group Program
WOS关键词	TUMOR-ASSOCIATED MACROPHAGES ; STEM-CELLS ; GROWTH ; MICROCARRIERS ; POLARIZATION ; COLLAGEN ; THERAPY
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000466612900029
资助机构	National Natural Science Foundation of China ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province ; Fundamental Research Funds for the Central Universities ; Max Planck Society International Partner Group Program
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/28146]
专题	中国科学院过程工程研究所
通讯作者	Bai, Shuo; Yin, Jian
作者单位	1.Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Di,Wang, Meiyue,Wang, Anhe,et al. Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2019,572:266-273.
APA	Wang, Di.,Wang, Meiyue.,Wang, Anhe.,Li, Jieling.,Li, Xin.,...&Yin, Jian.(2019).Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,572,266-273.
MLA	Wang, Di,et al."Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 572(2019):266-273.