Simultaneous size characterization and mass quantification of the in vivo core-biocorona structure and dissolved species of silver nanoparticles

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Simultaneous size characterization and mass quantification of the in vivo core-biocorona structure and dissolved species of silver nanoparticles
	Liu, Jingfu; Zhou, Xiaoxia; Dong, Lijie; Hu, Ligang; Yin, Yongguang
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2018
卷号	63 页码:227-235
关键词	Silver nanoparticle In vivo study Size characterization Mass quantification Size exclusion chromatography
ISSN号	1001-0742
文献子类	Article
英文摘要	Size characterization of silver nanoparticles with biomolecule corona (AgNP@BCs) and mass quantification of various silver species in organisms are essential for understanding the in vivo transformation of AgNPs. Herein, we report a versatile method that allows simultaneous determination of the size of AgNP@BCs and mass concentration of various silver species in rat liver. Both particulate and ionic silver were extracted in their original forms fromthe organs by alkaline digestion, and analyzed by size exclusion chromatography combined with inductively coupled plasma mass spectrometry (SEC-ICP-MS). While the silver mass concentrations were quantified by ICP-MS with a detection limit of 0.1 mu g/g, the effective diameter of AgNP@BCs was determined based on the retention time in SEC separation with size discrimination of 0.6-3.3 nm. More importantly, we found that the BC thickness of AgNP@BCs is core size independent, and a linear correlation was found between the effective diameter and core diameter of AgNP@BCs in extracted tissues, whichwas used to calibrate the core diameter with standard deviations in the range of 0.2-1.1 nm. The utility of this strategy was demonstrated through application to rat livers in vivo. Our method is powerful for investigating the transformation mechanism of AgNPs in vivo. (C) 2017 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/41037]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, Jingfu,Zhou, Xiaoxia,Dong, Lijie,et al. Simultaneous size characterization and mass quantification of the in vivo core-biocorona structure and dissolved species of silver nanoparticles[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2018,63:227-235.
APA	Liu, Jingfu,Zhou, Xiaoxia,Dong, Lijie,Hu, Ligang,&Yin, Yongguang.(2018).Simultaneous size characterization and mass quantification of the in vivo core-biocorona structure and dissolved species of silver nanoparticles.JOURNAL OF ENVIRONMENTAL SCIENCES,63,227-235.
MLA	Liu, Jingfu,et al."Simultaneous size characterization and mass quantification of the in vivo core-biocorona structure and dissolved species of silver nanoparticles".JOURNAL OF ENVIRONMENTAL SCIENCES 63(2018):227-235.