Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography
	Li, Yiling; He, Bin; Hu, Ligang; Huang, Xiu; Yun, Zhaojun; Liu, Runzeng; Zhou, Qunfang; Jiang, Guibin
刊名	TALANTA
	2018-02-01
卷号	178 页码:811-817
关键词	Immobilized mercury ion affinity chromatography Mercury Proteins SK-N-SH cell
ISSN号	0039-9140
文献子类	Article
英文摘要	Metal-binding proteins play important roles in biological functions of metals. However, only very limited mercury-binding proteins with high abundance were characterized in cells or organisms. Characterization of mercury-binding proteins in proteome-wide is important for elucidating mechanisms of mercury toxicity comprehensively. In this study, a method based on immobilized mercury ion affinity chromatography was developed for identification of putative mercury-binding proteins. The method was then successfully applied to profile mercury-binding proteins in human neuroblastoma SK-N-SH cells. In total; 38 proteins were identified as mercury-binding proteins, in which most of them were uncharacterized to associate with mercury in cells. The identified mercury-binding proteins did not show obvious relevance to protein abundance and were mainly involved in protein processing in endoplasmic reticulum, protein folding, and cytoskeleton organization. The newly built metalloproteomic approach provided valuable information on the possible molecular mechanisms and protein candidates for mercury transport and toxicity.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40897]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Li, Yiling,He, Bin,Hu, Ligang,et al. Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography[J]. TALANTA,2018,178:811-817.
APA	Li, Yiling.,He, Bin.,Hu, Ligang.,Huang, Xiu.,Yun, Zhaojun.,...&Jiang, Guibin.(2018).Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography.TALANTA,178,811-817.
MLA	Li, Yiling,et al."Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography".TALANTA 178(2018):811-817.