Analysis of microbial abundance and community composition in esophagus and intestinal tract of wild veined rapa whelk (Rapana venosa) by 16S rRNA gene sequencing

doi:10.2323/jgam.2017.11.003

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Analysis of microbial abundance and community composition in esophagus and intestinal tract of wild veined rapa whelk (Rapana venosa) by 16S rRNA gene sequencing
	Song, Hao 1,2,3; Yu, Zheng-Lin 1,2,3; Yang, Mei-Jie 1,2,3; Zhang, Tao1,2 ; Wang, Hai-Yan 1,2
刊名	JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
	2018
卷号	64 期号:4 页码:158-166
关键词	bacterial digestive tract gastropod Rapana venosa
ISSN号	0022-1260
DOI	10.2323/jgam.2017.11.003
通讯作者	Zhang, Tao(tzhang@qdio.ac.cn) ; Wang, Hai-Yan(haiyanwang@qdio.ac.cn)
英文摘要	The rapa whelk Rapana venosa is a commercially important gastropod in China, but a major invader worldwide. There is little information on the bacterial community composition in the digestive system of this species, despite the microflora has important roles in digestion, nutrition, disease resistance, and immune response. The present study investigated the bacterial flora community in the esophagus and intestinal tract of wild R. venosa by 16S rRNA gene sequencing. The esophagus and intestine had 1175 and 526 unique operational taxonomic units, respectively, with 616 common to both tissues. The 10 most highly represented microbial genera in the esophagus were Mycoplasma, Bifidobacterium, Escherichia, Shewanella, Vibrio, Lactobacillus, Octadecabacter, Enterococcus, Streptococcus, and Sphingomonas, which accounted for 41.35% of microbes. In the intestine, the most abundant genera were Mycoplasma, Bifidobacterium, Escherichia, Shewanella, Vibrio, Psychrilyobacter, Peptoniphilus, Sporobacterium, Octadecabacter, and Mobiluncusin (representing 62.74% of total microbes). A linear discriminant analysis, coupled with effect size, revealed that 31 taxa were differentially represented in esophagus and intestine bacterial communities. These results demonstrate the diversity of microbiota in the esophagus and intestinal tract of R. venosa and provide a basis for investigations into the physiological and immunological roles of these microorganisms in whelk.
资助项目	National Natural Science Foundation of China[31572636] ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Primary Research & Development Plan of Shandong Province[2016ZDJS06A02] ; industry leading talents project of Taishan scholars ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020703]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	MICROBIOL RES FOUNDATION
WOS记录号	WOS:000445602300002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/157609]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao; Wang, Hai-Yan
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Song, Hao,Yu, Zheng-Lin,Yang, Mei-Jie,et al. Analysis of microbial abundance and community composition in esophagus and intestinal tract of wild veined rapa whelk (Rapana venosa) by 16S rRNA gene sequencing[J]. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,2018,64(4):158-166.
APA	Song, Hao,Yu, Zheng-Lin,Yang, Mei-Jie,Zhang, Tao,&Wang, Hai-Yan.(2018).Analysis of microbial abundance and community composition in esophagus and intestinal tract of wild veined rapa whelk (Rapana venosa) by 16S rRNA gene sequencing.JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,64(4),158-166.
MLA	Song, Hao,et al."Analysis of microbial abundance and community composition in esophagus and intestinal tract of wild veined rapa whelk (Rapana venosa) by 16S rRNA gene sequencing".JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 64.4(2018):158-166.