A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1

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	A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1
	Wang, M.1; Wang, B.1; Liu, M.1; Jiang, K.1; Wang, L.1,2
刊名	ISJ-INVERTEBRATE SURVIVAL JOURNAL
	2017
卷号	14 页码:453-463
关键词	Litopenaeus vannamei macrophage migration inhibitory factor innate immunity
ISSN号	1824-307X
通讯作者	Wang, L.(wanglei@qdio.ac.cn)
英文摘要	Macrophage migration inhibitory factor (MIF) was an ancient cytokine and involved in innate immunity of vertebrates and invertebrates. In the present study, a novel MIF homologue (designated as LvMIF2) has been cloned from the Pacific white shrimp Litopenaeus vannamei via rapid amplification of cDNA ends (RACE) technique. The full-length cDNA sequence of LvMIF2 was 555 bp and contained a 97 bp 5' untranslated region (UTR) and a 3' UTR of 110 bp, and an open reading frame (ORF) of 348 bp which coded 115 amino acids. Quantitative realtime PCR (qRT-PCR) analysis indicated that LvMIF2 was constitutively expressed in all the tested tissues, including eyestalk, gill, gonad, heart, hemocytes, hepatopancreas, intestine, muscle, nerve and stomach, with the highest mRNA expression level in hemocytes and hepatopancreas. After Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge, the mRNA expression levels of LvMIF2 in hemocytes and hepatopancreas were all sharply upregulated. Comparative sequence and transcription analysis between the previously identified LvMIF1 and LvMIF2 revealed that both LvMIF1 and LvMIF2 might play crucial and functionally differentiated roles in shrimp innate immune responses to bacterial and viral stimulation.
资助项目	Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002]
WOS研究方向	Immunology ; Zoology
语种	英语
出版者	INVERTEBRATE SURVIVAL JOURNAL
WOS记录号	WOS:000416558800003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/157275]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Wang, M.,Wang, B.,Liu, M.,et al. A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2017,14:453-463.
APA	Wang, M.,Wang, B.,Liu, M.,Jiang, K.,&Wang, L..(2017).A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1.ISJ-INVERTEBRATE SURVIVAL JOURNAL,14,453-463.
MLA	Wang, M.,et al."A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1".ISJ-INVERTEBRATE SURVIVAL JOURNAL 14(2017):453-463.