Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites

doi:10.1016/j.fsi.2018.09.011

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites
	Li, Meng1,2 ; Huang, Qian 1,2,3; Wang, Jinfeng1,2,3 ; Li, Caiwen 1,2,4
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018-12-01
卷号	83 页码:134-139
关键词	Marine crustacean Parasite Innate immunity microRNA Host-parasite interaction
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.09.011
通讯作者	Li, Caiwen(caiwenli@live.com)
英文摘要	Latest studies have indicated that microRNAs (miRNAs) play important roles in defending against bacterial and viral infections in marine crustacean, whereas little is known regarding the immunological roles of crustacean miRNAs in response to parasitic infection. To further reveal the host-parasite interactions between the parasitic dinoflagellate Hematodinium and its crustacean hosts, we applied the high-throughput sequencing technology to identify and characterize miRNAs in the Chinese swimming crab Portunus trituberculatus challenged with the Hematodinium parasite at a timescale of 16 days (d). A total of 168 miRNAs were identified and 51 miRNAs were differentially expressed in the hepatopancreas tissues of affected hosts. Eleven of the differentially expressed miRNAs were selected and verified by the quantitative real-time RT-PCR (qRT-PCR), manifesting the consistency between the high throughout sequencing and qRT-PCR assays. Further analysis of the putative target genes indicated that various immune-related pathways (e.g. endocytosis, Fc gamma R-mediated phagocytosis, lysosome, ECM-receptor interaction, complement and coagulation cascades, antigen processing and presentation, focal adhesion, etc.) and signal transduction pathways (e.g. JAK-STAT signaling pathway, MAPK signaling pathway, p53 signaling pathway, etc.) were mediated by the differentially expressed miRNAs. The results presented fundamental knowledge on the immunological roles of crustacean miRNAs and contributed to the better understanding of hosts' miRNAs-mediated immunity against the parasitic infection.
资助项目	General Program of National Nature Science Foundation of China (NSFC)[41676102] ; CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows[2016LH0034] ; China Postdoctoral Science Foundation[2016M600561]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000448096200015
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/156532]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Li, Caiwen
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Meng,Huang, Qian,Wang, Jinfeng,et al. Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites[J]. FISH & SHELLFISH IMMUNOLOGY,2018,83:134-139.
APA	Li, Meng,Huang, Qian,Wang, Jinfeng,&Li, Caiwen.(2018).Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites.FISH & SHELLFISH IMMUNOLOGY,83,134-139.
MLA	Li, Meng,et al."Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites".FISH & SHELLFISH IMMUNOLOGY 83(2018):134-139.