A Supercomplex, of Approximately 720 kDa and Composed of Both Photosystem Reaction Centers, Dissipates Excess Energy by PSI in Green Macroalgae Under Salt Stress

doi:10.1093/pcp/pcy201

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A Supercomplex, of Approximately 720 kDa and Composed of Both Photosystem Reaction Centers, Dissipates Excess Energy by PSI in Green Macroalgae Under Salt Stress
	Gao, Shan 1,2,3; Chi, Zhen 4,5; Chen, Hailong 4; Zheng, Zhenbing 1,2,3,5; Weng, Yuxiang 4; Wang, Guangce 1,2,3
刊名	PLANT AND CELL PHYSIOLOGY
	2019
卷号	60 期号:1 页码:166-175
关键词	Intertial macroalgae Photosystems Salt stress Supercomplex Thylakoid membrane Ulva prolifera
ISSN号	0032-0781
DOI	10.1093/pcp/pcy201
通讯作者	Weng, Yuxiang(yxweng@iphy.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	The thylakoid membranes of plants play a critical role in electron transfer and energy fixation, and are highly dynamic. So far, studies on the thylakoid membranes have mainly focused on microalgae and higher plants, yet very little information is available on the macroalgal thylakoids. Here, we studied the structure and organization of the thylakoid membranes in Ulva prolifera, a representative species of the green macroalgae. We found that U. prolifera had few but long loosely stacked membranes which lack the conventional grana found in higher plants. However, the thylakoid membrane complexes demonstrate lateral heterogeneity. Moreover, we found a supercomplex composed of PSII, light-harvesting complex II (LHCII) and PSI from U. prolifera under salt stress. The supercomplex is approximately 720 kDa, and includes the two important photoprotection proteins, the PSII S subunit (PsbS) and the light-harvesting complex stress-related protein (LhcSR), as well as xanthophyll cycle pigments (violaxanthin, antheraxanthin and zeaxanthin). Time-resolved fluorescence analysis suggested that, in the supercomplex, excitation energy could efficiently be transferred from PSII to PSI, even when PSII was inhibited, a function which disappeared when the supercomplex was incubated in mild detergent. We suggest that the supercomplex might be an important mechanism to dissipate excess energy by PSI in green macroalgae under salt stress.
资助项目	National Natural Science Foundation of China[41606161] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41476140] ; Distinguished Young Scientists Research Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KLEMB-DYS02] ; China Agriculture Research System[CARS-50] ; Key Laboratory of Algal Biology Institute of Hydrobiology Chinese Academy of Sciences[2017-003] ; Project of Science and Technology Bureau of Nantong[MS12017025-4]
WOS研究方向	Plant Sciences ; Cell Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000459633500016
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/155581]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Weng, Yuxiang; Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gao, Shan,Chi, Zhen,Chen, Hailong,et al. A Supercomplex, of Approximately 720 kDa and Composed of Both Photosystem Reaction Centers, Dissipates Excess Energy by PSI in Green Macroalgae Under Salt Stress[J]. PLANT AND CELL PHYSIOLOGY,2019,60(1):166-175.
APA	Gao, Shan,Chi, Zhen,Chen, Hailong,Zheng, Zhenbing,Weng, Yuxiang,&Wang, Guangce.(2019).A Supercomplex, of Approximately 720 kDa and Composed of Both Photosystem Reaction Centers, Dissipates Excess Energy by PSI in Green Macroalgae Under Salt Stress.PLANT AND CELL PHYSIOLOGY,60(1),166-175.
MLA	Gao, Shan,et al."A Supercomplex, of Approximately 720 kDa and Composed of Both Photosystem Reaction Centers, Dissipates Excess Energy by PSI in Green Macroalgae Under Salt Stress".PLANT AND CELL PHYSIOLOGY 60.1(2019):166-175.