Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida

doi:10.1007/s10811-018-1576-1

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida
	Li, Jing1,2 ; Huang, Haishu 1,2,3; Shan, Tifeng 1,2; Pang, Shaojun 1,2
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2019-02-01
卷号	31 期号:1 页码:787-793
关键词	Reference genes qRT-PCR geNorm NormFinder Phaeophyta Undaria pinnatifida
ISSN号	0921-8971
DOI	10.1007/s10811-018-1576-1
通讯作者	Pang, Shaojun(sjpang@ms.qdio.ac.cn)
英文摘要	Quantitative real-time PCR (qRT-PCR) has become widely used method for detecting gene expression of an organism at different developmental stages or under various stressful environmental conditions. Finding reference genes is a prerequisite in this process. In this research, we investigated the expression stability of eight candidate genes in the brown alga Undaria pinnatifida, including EF1 (elongation factor 1-alpha), eEF1 (eukaryotic elongation factor-1 B beta), RPL35 (60S ribosomal protein L35-3), GAPDH (glyceraldehyde-3-phosphatedehydrogenase), RPS17 (40S ribosomal protein S17), LHCP (light-harvesting complex protein), ribH (6,7-dimethyl-8-ribityllumazine synthase), and ilvC (ketol-acid reductoisomerase). The haploid male and female gametophytes at different stages, as well as the diploid sporophytes treated with different abiotic factors were analyzed by geNorm and NormFinder software. It was found that eEF1 and ribH could be used as the most stable reference genes in studies of gender or developmental gene expression in the gametophytes. As for the detection of gene expression in the sporophytes, eEF1 and ribH were recommended as the most suitable reference genes for irradiance treatment, eEF1 and RPL35 for temperature treatment, while EF1 and LHCP for nutrition treatment. These results will enable us to go further for quantifying gene expression study at transcript level in U. pinnatifida.
资助项目	National Natural Science Foundation of China[41476141] ; National Natural Science Foundation of China[31702365] ; National Natural Science Foundation of China[41676128] ; Sino-German Science Center[GZ 1080] ; China Agriculture Research System[CARS-50] ; National Key Technology Support Program[2015BAD13B05] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470] ; Taishan Scholar Program of Shandong Province ; Huiquan Scholar Program of Institute of Oceanology, Chinese Academy of Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000458698200069
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/155412]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Pang, Shaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Huang, Haishu,Shan, Tifeng,et al. Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(1):787-793.
APA	Li, Jing,Huang, Haishu,Shan, Tifeng,&Pang, Shaojun.(2019).Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida.JOURNAL OF APPLIED PHYCOLOGY,31(1),787-793.
MLA	Li, Jing,et al."Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida".JOURNAL OF APPLIED PHYCOLOGY 31.1(2019):787-793.