Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts

doi:10.1016/j.soilbio.2017.10.033

	Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts
	Wang, Xiao Lin 1; Cui, Wen Jing 1; Feng, Xue Ying 1; Zhong, Zhi Ming 2; Li, Ying 1; Chen, Wen Xin 1; Chen, Wen Feng 1; Shao, Xiao Ming 3; Tian, Chang Fu 1
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2018
卷号	116 页码:340-350
关键词	Alfalfa Nodule Rhizobia Rhizosphere rpoB
ISSN号	0038-0717
DOI	10.1016/j.soilbio.2017.10.033
通讯作者	Shao, Xiao Ming(shaoxm@cau.edu.cn) ; Tian, Chang Fu(cftian@cau.edu.cn)
英文摘要	Alfalfa (Medicago sativa L.) is an important leguminous forage crop, which is hypothesized to select compatible rhizobial partners from rhizosphere to form nitrogen-fixing nodules. Here we investigated the diversity of rhizobia from both nodules and rhizosphere of alfalfa from farmlands and natural ecosystems along the Yarlung Zangbo River valley in the Tibetan Plateau. 581 nodule isolates were characterized using BOX-A1R PCR fingerprinting, sequences of rpoB and nodulation gene nodC. They belong to four rpoB haplotypes of Sinorhizobium meliloti (tpoB-I, 473 isolates; tpoB-II, 95; rpoB-III, 1; rpoB-IV, 10), Sinorhizobium medicae (1 isolate) and Rhizobium sp. (1 isolate). Re-inoculation experiments of the 32 representative strains suggested a great variation in their symbiotic performance on alfalfa. High-throughput sequencing of rpoB for 34 rhizosphere samples uncovered that the predominant rpoB-I in nodules also dominated among fourteen rpoB haplotypes of S. meliloti in rhizosphere soils. In addition to S. meliloti, more than 40 rhizobial species documented as microsymbionts of other legumes were also identified. Although a very low level of nucleotide diversity for S. meliloti was found among sampling sites, multivariate statistical analysis demonstrated a significant differentiation of total rhizobial community between farmlands and natural ecosystems. Moreover, edaphic conditions, especially pH and nitrogen content, were revealed as important deterministic factors shaping the observed beta-diversity of rhizobial community. Taken together, these results demonstrated that the rhizospheric pool of rhizobial germplasms was significantly affected by land tillage compared to that of natural ecosystems, while a predominant population of S. meliloti was selected by alfalfa.
资助项目	Program for New Century Excellent Talents in University[NCET-13-0561] ; National Basic Research Program of China (973 program)[2015CB158300] ; Chinese Universities Scientific Fund[2017SY003] ; Chinese Universities Scientific Fund[2017TC022] ; Key Research and Transferability Program in the Tibetan Autonomous Region[ZH20170001] ; State Key Laboratory of Agrobiotechnology ; Ministry of Science and Technology[2013BAD30B02-4]
WOS关键词	SINORHIZOBIUM-MELILOTI ; GENETIC DIVERSITY ; MEDICAGO-TRUNCATULA ; SOYBEAN RHIZOSPHERE ; NITROGEN-FIXATION ; FIELD CONDITIONS ; TUNISIAN SOILS ; SEQUENCE DATA ; SP. NOV. ; LEGUMES
WOS研究方向	Agriculture
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000419417900037
资助机构	Program for New Century Excellent Talents in University ; National Basic Research Program of China (973 program) ; Chinese Universities Scientific Fund ; Key Research and Transferability Program in the Tibetan Autonomous Region ; State Key Laboratory of Agrobiotechnology ; Ministry of Science and Technology
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/56896]
专题	中国科学院地理科学与资源研究所
通讯作者	Shao, Xiao Ming; Tian, Chang Fu
作者单位	1.China Agr Univ, Coll Biol Sci, Rhizobium Res Ctr, MOA Key Lab Soil Microbiol,State Key Lab Agrobiot, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China 3.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiao Lin,Cui, Wen Jing,Feng, Xue Ying,et al. Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts[J]. SOIL BIOLOGY & BIOCHEMISTRY,2018,116:340-350.
APA	Wang, Xiao Lin.,Cui, Wen Jing.,Feng, Xue Ying.,Zhong, Zhi Ming.,Li, Ying.,...&Tian, Chang Fu.(2018).Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts.SOIL BIOLOGY & BIOCHEMISTRY,116,340-350.
MLA	Wang, Xiao Lin,et al."Rhizobia inhabiting nodules and rhizosphere soils of alfalfa: A strong selection of facultative microsymbionts".SOIL BIOLOGY & BIOCHEMISTRY 116(2018):340-350.