Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance

doi:10.1016/j.gene.2018.04.056

	Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance
	Xiang, N (Xiang, Nan); Yang, YP (Yang, Yongping)1,2,3; Chen, Q (Chen, Qian); Yang, SH (Yang, Shihai); Kong, XX (Kong, Xiangxiang); Wang, CT (Wang, Chuntao); Yang, YQ (Yang, Yunqiang)1,2,3
刊名	GENE
	2018-07-30
卷号	665 期号:0 页码:41-48
关键词	Gene-expression Phaseolus-vulgaris Pip1 Subfamily Water Channel Tobacco Overexpression Protein Identification Dehydrin Improves
ISSN号	0378-1119
DOI	10.1016/j.gene.2018.04.056
英文摘要	Stipa purpurea is widely distributed on the Tibetan Plateau, and has high drought resistance. Plasma membrane intrinsic proteins are a type of aquaporin. They regulate the movement of water and are associated with plant protective reactions to biotic and abiotic stresses. We characterized a plasma membrane intrinsic protein from S. purpurea (SpPIP1) and elucidated its role in molecular aspects of the plant's response to drought stress. The full-length open reading frame of SpPIP1 was 870 bp and encoded 289 amino acids. The transcript level of SpPIP1 was higher in the root of S. purpurea than in the flower, leaf and stem. The level of SpPIP1 transcript increased significantly when treated with drought treatment. Subcellular localization result showed that SpPIP1 was localized in the plasma membrane. Ectopic expression of SpPIP1 in Arabidopsis thaliana resulted in plants with higher tolerance to drought treatment. SpPIP1 of S. purpurea may mediate plant response to arid environments.
学科主题	生态学
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000435621900006
内容类型	期刊论文
源URL	[http://ir.itpcas.ac.cn/handle/131C11/8590]
专题	青藏高原研究所_图书馆
通讯作者	Yang, YP (Yang, Yongping); Yang, YQ (Yang, Yunqiang)
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China; 2.Chinese Acad Sci, Inst Tibetan Plateau Res Kunming, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China. 3.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China;
推荐引用方式 GB/T 7714	Xiang, N ,Yang, YP ,Chen, Q ,et al. Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance[J]. GENE,2018,665(0):41-48.
APA	Xiang, N .,Yang, YP .,Chen, Q .,Yang, SH .,Kong, XX .,...&Yang, YQ .(2018).Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance.GENE,665(0),41-48.
MLA	Xiang, N ,et al."Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance".GENE 665.0(2018):41-48.