The APOBEC3 family is a series antiviral factors that inhibit the replication of many viruses, such as HIV-1 andHBV. Tree shrews (Tupaia belangeri) possess great potential as an animal model for human diseases and ther-apeutic responses. However, the APOBEC3 family is unknown in tree shrews. Recent work has showed thepresence of the APOBEC3 family in tree shrews. In this work, the cDNA sequences of five APOBEC3 memberswere identified in tree shrews, namely, tsAPOBEC3A, -3C, -3F, -3G and -3H. The results showed that their se-quences encoded a zinc (Z)-coordinating-domain as a characteristic of APOBEC3 proteins. Phylogenetic analysisrevealed that the tree shrew APOBEC3 (tsAPOBEC3) genes have occurred independently and that they areclustered with other mammalian APOBEC3 members. Transcript expression analysis indicated that tsAPOBEC3genes are constitutively expressed, and high in immune-related tissues. tsAPOBEC3 gene expression was up-regulated in hepatocytes and PBMCs by IFN-α stimulation. Finally, tsAPOBEC3 proteins could edit both sides ofDNA by inserting G → A and C → T hypermutations. Overall, the results suggest that the tsAPOBEC3 familycould play a key role in defense immunity through distinct editing mechanisms. Our results provided insightsinto the genetic basis for the development of a tree shrew model for studying viral infection. Future studies willfocus on deepening our understanding on the antiviral functions of these editing enzymes in tree shrew.