Calcineurin Antagonizes AMPK to Regulate Lipolysis in Caenorhabditis elegans

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	Calcineurin Antagonizes AMPK to Regulate Lipolysis in Caenorhabditis elegans
	Liang B*1; Diao ZQ 1,2; Xie CS 1,3; Wang YL 1; liangb@mail.kiz.ac.cn
刊名	Molecules
	2017
卷号	22 期号:X 页码:e1062
关键词	Calcineurin Tacrolimus Amp-activated Protein Kinase (Ampk) Triglyceride Lipase Atgl Lipolysis
英文摘要	Calcineurin is a calcium- and calmodulin-dependent serine/threonine protein phosphatase, and the target of immunosuppressive agent tacrolimus (TAC). The dysfunction of calcineurin, or clinical applications of tacrolimus, have been reported to be associated with dyslipidemia. The underlying mechanisms of calcineurin and tacrolimus in lipid metabolism are largely unknown. Here, we showed that mutations of tax-6 and cnb-1, which respectively encode the catalytic subunit and the regulatory subunit of calcineurin, together with tacrolimus treatment, consistently led to decreased fat accumulation and delayed growth in the nematode Caenorhabditis elegans. In contrast, disruption of the AMP-activated protein kinase (AMPK) encoded by aak-1 and aak-2 reversed the above effects in worms. Moreover, calcineurin deficiency and tacrolimus treatment consistently activated the transcriptional expression of the lipolytic geneatgl-1, encoding triglyceride lipase. Furthermore, RNAi knockdown of atgl-1 recovered the decreased fat accumulation in both calcineurin deficient and tacrolimus treated worms. Collectively, our results reveal that immunosuppressive agent tacrolimus and their target calcineurin may antagonize AMPK to regulate ATGL and lipolysis, thereby providing potential therapy for the application of immunosuppressive agents.
语种	英语
资助机构	This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13030600), National Natural Science Foundation of China (31600963, 31671230, 31171134, U1202223, 31401014, 31460268), Yunnan Natural Science Foundation (2013FA042), and Yunnan Provincial Science, Technology Department (2014HB022), and Yunnan Oversea High-level Talents Program (2015HA040). ; This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13030600), National Natural Science Foundation of China (31600963, 31671230, 31171134, U1202223, 31401014, 31460268), Yunnan Natural Science Foundation (2013FA042), and Yunnan Provincial Science, Technology Department (2014HB022), and Yunnan Oversea High-level Talents Program (2015HA040). ; This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13030600), National Natural Science Foundation of China (31600963, 31671230, 31171134, U1202223, 31401014, 31460268), Yunnan Natural Science Foundation (2013FA042), and Yunnan Provincial Science, Technology Department (2014HB022), and Yunnan Oversea High-level Talents Program (2015HA040). ; This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13030600), National Natural Science Foundation of China (31600963, 31671230, 31171134, U1202223, 31401014, 31460268), Yunnan Natural Science Foundation (2013FA042), and Yunnan Provincial Science, Technology Department (2014HB022), and Yunnan Oversea High-level Talents Program (2015HA040).
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/11790]
专题	昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_脂类代谢与疾病
通讯作者	liangb@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.School of Life Sciences, Anhui University, Hefei 230601, China 3.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650204, China
推荐引用方式 GB/T 7714	Liang B*,Diao ZQ,Xie CS,et al. Calcineurin Antagonizes AMPK to Regulate Lipolysis in Caenorhabditis elegans[J]. Molecules,2017,22(X):e1062.
APA	Liang B*,Diao ZQ,Xie CS,Wang YL,&liangb@mail.kiz.ac.cn.(2017).Calcineurin Antagonizes AMPK to Regulate Lipolysis in Caenorhabditis elegans.Molecules,22(X),e1062.
MLA	Liang B*,et al."Calcineurin Antagonizes AMPK to Regulate Lipolysis in Caenorhabditis elegans".Molecules 22.X(2017):e1062.