Chemically Bonded Polyacrylamide via Thiol-Ene Click Chemistry as Separation Materials for Hydrophilic Interaction Liquid Chromatography

doi:10.1016/S1872-2040(15)60863-5

	Chemically Bonded Polyacrylamide via Thiol-Ene Click Chemistry as Separation Materials for Hydrophilic Interaction Liquid Chromatography
	Yang Duo 1,2; Yu Dong-Ping 2; Dong Xue-Fang 2; Shen Ai-Jin 2; Jin Gao-Wa 2; Guo Zhi-Mou 2; Yan Jing-Yu 2; Liu Ming-Yang 1,2,3; Liang Xin-Miao 2
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2015-10-01
卷号	43 期号:10 页码:1439-1444
关键词	Reversible Addition Fragmentation Chain Transfer Polyacrylamide "thiol-ene" Click Chemistry Hydrophilic Interaction Liquid Chromatography
ISSN号	0253-3820
DOI	10.1016/S1872-2040(15)60863-5
文献子类	Article
英文摘要	Thiol-ene click chemistry was applied to immobilize polyacrylamide onto alkenyl-modified silica to prepare a novel stationary phase for hydrophilic interaction liquid chromatography. Polyacrylamide was synthesized by reversible addition-fragmentation chain transfer reaction. Alkenyl functionalities were introduced onto the silica by the reaction of silica with the reaction product of 3-isocyanate opropyl trimethoxy silane, allylamine and pyridine in toluene to obtain alkenyl-modified silica (EUS, E-thiol-ene, U-urea, S-silica). The alkenyl-modified silica was then reacted with polyacrylamide in methanol at 55 degrees C for 48 h in the presence of 2,2-azo diisobutyl amidine hydrochloride to prepare a novel stationary phase (TE-UPAM, TE-thiol-ene, U-urea, PAM-polyacrylamide), for hydrophilic interaction liquid chromatography. This new stationary phase "TE-UPAM" was characterized by element analysis and infrared spectra, and the results showed that, compared with EUS, the carbon content of TE-UPAM increased and the characteristic peaks of multiple amide existed at 1636 and 1570 cm(-1). The effects of water content, salt concentration and pH of the mobile phase on the retention time of polar compounds were investigated. The results showed that the retention time of polar compounds decreased with the increase of water content. In contrast with acidic polar compounds, the retention time of neutral and basic polar compounds increased with the increase of salt concentration, and the retention time of basic polar compounds decreased with the decrease of pH value in the range of 3.3-4.8. The novel stationary phase behaved better than traditional silica stationary phase in the separation of uridine and bases. In addition, good separation of oligosaccharides by the novel stationary phase was also achieved, indicating great potential in the separation of polar compounds.
WOS关键词	STATIONARY PHASES ; POLAR COMPOUNDS ; PEPTIDES ; SURFACE ; POLYMERIZATION ; NANOPARTICLES ; COLUMNS ; FACILE
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000365738500001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171594]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Guo Zhi-Mou
作者单位	1.Dalian Jiaotong Univ, Coll Environm & Chem Engn, Dalian 116028, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Liaoning Entry Exit Inspect & Quarantine Bur, Ctr Tech, Dalian 116001, Peoples R China
推荐引用方式 GB/T 7714	Yang Duo,Yu Dong-Ping,Dong Xue-Fang,et al. Chemically Bonded Polyacrylamide via Thiol-Ene Click Chemistry as Separation Materials for Hydrophilic Interaction Liquid Chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2015,43(10):1439-1444.
APA	Yang Duo.,Yu Dong-Ping.,Dong Xue-Fang.,Shen Ai-Jin.,Jin Gao-Wa.,...&Liang Xin-Miao.(2015).Chemically Bonded Polyacrylamide via Thiol-Ene Click Chemistry as Separation Materials for Hydrophilic Interaction Liquid Chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,43(10),1439-1444.
MLA	Yang Duo,et al."Chemically Bonded Polyacrylamide via Thiol-Ene Click Chemistry as Separation Materials for Hydrophilic Interaction Liquid Chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 43.10(2015):1439-1444.