Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells

doi:10.1155/2016/9291013

	Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells
	Lian, Jianchun 3,4; Lv, Shijie 1; Liu, Chang 3,5; Liu, Yang 3; Wang, Shujun 3; Guo, Xin 3; Nan, Feng 2; Yu, Hua 1; He, Xin 4; Sun, Guangwei 3
刊名	STEM CELLS INTERNATIONAL
	2016
ISSN号	1687-966X
DOI	10.1155/2016/9291013
文献子类	Article
英文摘要	Background and Objective. It is important to guarantee the quality of stem cells. Serial passage is the main approach to expand stem cells. This study evaluated effects of serial passage on the biological characteristics of human umbilical cord Wharton's jelly-derived MSCs (WJ MSCs). Methods. Biological properties of WJ MSCs in the early (less than 10 passages, P10), middle (P11-20), and late (more than P20) phases including cell proliferation, cell cycle, phenotype, senescence, oncogene expression, stemness marker expression, and differentiation capacity were evaluated using flow cytometry, real-time PCR, immunocytofluorescence, and western blot. Results. It was found that there were no significant differences in cell proliferation, cell cycle, phenotype, and stemness marker expression in different phases. However, the expression of senescence-related gene, p21, and oncogene, c-Myc, was significantly upregulated in the late phase, which had close relations with the obviously increased cell senescence. Moreover, cardiac differentiation capability of WJ MSCs decreased whereas the propensity for neural differentiation increased significantly in the middle phase. Conclusions. This study reveals that WJ MSCs in the early and middle phases are relatively stable, and effect of serial passage on the lineage-specific differentiation should be considered carefully.
WOS关键词	BONE-MARROW ; GENE-EXPRESSION ; PROLIFERATION ; ACTIVATION ; PATHWAY ; ADIPOSE
WOS研究方向	Cell Biology
语种	英语
出版者	HINDAWI PUBLISHING CORP
WOS记录号	WOS:000369722900001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171362]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Yang
作者单位	1.Dalian Matern & Child Healthcare Hosp, Dalian 116033, Peoples R China 2.Dalian Med Univ, Hosp 2, Dept Orthoped, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biotechnol, Dalian 116023, Peoples R China 4.Dalian Med Univ, Lab Med Coll, Dalian 116044, Peoples R China 5.Uni Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lian, Jianchun,Lv, Shijie,Liu, Chang,et al. Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells[J]. STEM CELLS INTERNATIONAL,2016.
APA	Lian, Jianchun.,Lv, Shijie.,Liu, Chang.,Liu, Yang.,Wang, Shujun.,...&Ma, Xiaojun.(2016).Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells.STEM CELLS INTERNATIONAL.
MLA	Lian, Jianchun,et al."Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells".STEM CELLS INTERNATIONAL (2016).