Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition

doi:10.1007/s11802-016-2847-6

	Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition
	Zhou Jiannan; Cao Xupeng; Wu Shuang; Xue Song
刊名	JOURNAL OF OCEAN UNIVERSITY OF CHINA
	2016-02-01
卷号	15 期号:1 页码:137-144
关键词	Chrysophyta Isochrysis Zhangjiangensis Nitrogen Deficiency Lipid Accumulation Reference Gene Selection
ISSN号	1672-5182
DOI	10.1007/s11802-016-2847-6
文献子类	Article
英文摘要	Isochrysis zhangjiangensis is a potential marine microalga for biodiesel production, which accumulates lipid under nitrogen limitation conditions, but the mechanism on molecular level is veiled. Quantitative real-time polymerase chain reaction (qPCR) provides the possibility to investigate the gene expression levels, and a valid reference for data normalization is an essential prerequisite for firing up the analysis. In this study, five housekeeping genes, actin (ACT), alpha-tubulin (TUA), beta-tubulin (TUB), ubiquitin (UBI), 18S rRNA (18S) and one target gene, diacylglycerol acyltransferase (DGAT), were used for determining the reference. By analyzing the stabilities based on calculation of the stability index and on operating the two types of software, geNorm and bestkeeper, it showed that the reference genes widely used in higher plant and microalgae, such as UBI, TUA and 18S, were not the most stable ones in nitrogen-stressed I. zhangjiangensis, and thus are not suitable for exploring the mRNA expression levels under these experimental conditions. Our results show that ACT together with TUB is the most feasible internal control for investigating gene expression under nitrogen-stressed conditions. Our findings will contribute not only to future qPCR studies of I. zhangjiangensis, but also to verification of comparative transcriptomics studies of the microalgae under similar conditions.
WOS关键词	REAL-TIME PCR ; MESSENGER-RNA ; RT-PCR ; CHLAMYDOMONAS-REINHARDTII ; HOUSEKEEPING GENES ; NITRATE TRANSPORTER ; TRANSCRIPT LEVELS ; QUANTIFICATION ; NORMALIZATION ; TRIACYLGLYCEROLS
WOS研究方向	Oceanography
语种	英语
出版者	OCEAN UNIV CHINA
WOS记录号	WOS:000371073400017
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171276]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xue Song
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhou Jiannan,Cao Xupeng,Wu Shuang,et al. Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2016,15(1):137-144.
APA	Zhou Jiannan,Cao Xupeng,Wu Shuang,&Xue Song.(2016).Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition.JOURNAL OF OCEAN UNIVERSITY OF CHINA,15(1),137-144.
MLA	Zhou Jiannan,et al."Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition".JOURNAL OF OCEAN UNIVERSITY OF CHINA 15.1(2016):137-144.