Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening

doi:10.1002/biot.201500183

	Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening
	Qin, Jianhua 3; Li, Yanfeng 3; Ma, Jingyun 1,2,3; Zhang, Xu 3; Liu, Yang 3; Yu, Haibo 1; Liu, Lianqing 1; Shi, Yang 3
刊名	BIOTECHNOLOGY JOURNAL
	2016
卷号	11 期号:1 页码:127-134
关键词	Drug Screening Hypoxia-inducible Factors (Hifs) Micro-patterning Multicellular Spheroids Phenotype Transition
ISSN号	1860-6768
DOI	10.1002/biot.201500183
文献子类	Article
英文摘要	3D multicellular spheroid models are of great value in the investigation of tumor biology and tumor responses to chemotherapy and radiation. To establish a mimicking tumor microenvironment in vitro, we developed a straightforward method by patterning hypoxic multicellular spheroids in a 3D matrix. The efficacy of this approach was evaluated by characterizing spheroid formation, invasive capability and phenotypic transition in aggressive human glioma cells. We observed enhanced cell proliferation, spheroid formation and invasive capability in U87 glioma cells transfected with hypoxia-inducible factors (HIFs) compared with non-treated cells. We also demonstrated that the overexpression of HIFs in hypoxic glioma cells may promote cell migration by epithelial-mesenchymal transition within the 3D matrix. Compared with conventional 3D culturing techniques, the simple operation, rapid prototyping, low cost and high throughput format of the micro-patterning method facilitates the characterization of cell proliferation, migration, phenotypic function and drug evaluation in physiologically relevant 3D microenvironments. This in vitro 3D system can recapitulate the physiologically relevant tumor microenvironment and is a promising method for 3D anti-tumor drug screening and the identification of novel targets for tumor invasion and angiogenesis.
WOS关键词	CELL-CULTURE SYSTEMS ; IN-VITRO ; MESENCHYMAL TRANSITION ; CANCER ; MODELS ; GROWTH ; IDENTIFICATION ; DISCOVERY ; INVASION ; 3-D
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000370002300012
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171198]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Qin, Jianhua
作者单位	1.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Qin, Jianhua,Li, Yanfeng,Ma, Jingyun,et al. Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening[J]. BIOTECHNOLOGY JOURNAL,2016,11(1):127-134.
APA	Qin, Jianhua.,Li, Yanfeng.,Ma, Jingyun.,Zhang, Xu.,Liu, Yang.,...&Shi, Yang.(2016).Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening.BIOTECHNOLOGY JOURNAL,11(1),127-134.
MLA	Qin, Jianhua,et al."Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening".BIOTECHNOLOGY JOURNAL 11.1(2016):127-134.