A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

doi:10.3892/or.2016.4742

	A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells
	Kan, Xiao-Xi 1; Li, Qi 1; Chen, Xi 1; Wang, Ya-Jie 1; Li, Yu-Jie 1; Yang, Qing 1; Xiao, Hong-Bin 2; Wang, Zhi-Xin 2; Chen, Ying 1; Weng, Xiao-Gang 1
刊名	ONCOLOGY REPORTS
	2016-06-01
卷号	35 期号:6 页码:3480-3488
关键词	Cell Cycle Arrest Hepatocarcinoma Cyclin B1 Cdk1 SteL.era Chamaejasme L.
ISSN号	1021-335X
DOI	10.3892/or.2016.4742
文献子类	Article
英文摘要	Currently, liver cancer is the sixth most prevalent cancer and the third most common cause of cancer-related death. However, effective chemotherapeutic drugs with low drug resistance and few side-effects for the clinical treatment of liver cancer are lacking. Therefore, the search for novel drugs to compensate for the defects of existing drugs is urgently needed. Herein, we successfully screened an extract named from Stellera chamaejasme L. (SCL), a historically confimed antitumor plant, through a novel extraction platform. In the present study, we firstly screened the anticancer effect of ESC by the sulforhodamine B (SRB) cell proliferation assay in a wide range of malignant cell lines, including A549, NCI-H157, NCI-H460, SK-HEP-1 and HepG2. With the highest inhibitory rate in hepatocarcinoma cells, we further identified the tumor-suppressive efficacy and the safety of ESC in an H22 hepatocarcinoma xenograft model in vivo. In a mechanistic study, flow cytometry and western blot analysis were performed to evaluate the effects of ESC on the induction of cell apoptosis, intervention of cell cycle distribution and its influence on key G2/M-phase regulators. The results showed that ESC significantly inhibited the cell growth of liver cancer cell lines. Accordingly, the tumor inhibition rate was also increased following ESC administration with little systemic toxicity in H22-transplanted mice. Mechanistically, ESC caused obvious G2/M-phase arrest in both the SK-HEP-1 and HepG2 cell lines without cell apoptosis. Furthermore, cyclin B1 was downregulated, while the phosphorylation level of CDK1 was increased in response to ESC treatment. All these data confirmed that ESC possesses potent anti-proliferative efficacy for hepatocarcinoma through the induction of cyclin-mediated cell cycle arrest. Thus, ESC is a promising candidate for hepatocarcinoma treatment in the future.
WOS关键词	HEPATOCELLULAR-CARCINOMA ; SYSTEMIC CHEMOTHERAPY ; ARTERIAL INFUSION ; LIVER RESECTION ; NATURAL-HISTORY ; CANCER ; PERSPECTIVE ; DOXORUBICIN ; EPIRUBICIN ; MANAGEMENT
WOS研究方向	Oncology
语种	英语
出版者	SPANDIDOS PUBL LTD
WOS记录号	WOS:000376550600040
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170996]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhu, Xiao-Xin
作者单位	1.China Acad Chinese Med Sci, Inst Chinese Mat Med, 16 Dong Zhi Men South St, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Kan, Xiao-Xi,Li, Qi,Chen, Xi,et al. A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells[J]. ONCOLOGY REPORTS,2016,35(6):3480-3488.
APA	Kan, Xiao-Xi.,Li, Qi.,Chen, Xi.,Wang, Ya-Jie.,Li, Yu-Jie.,...&Zhu, Xiao-Xin.(2016).A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells.ONCOLOGY REPORTS,35(6),3480-3488.
MLA	Kan, Xiao-Xi,et al."A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells".ONCOLOGY REPORTS 35.6(2016):3480-3488.