Environment-induced conformational and functional changes of L-2-haloacid dehalogenase

doi:10.1016/j.jbiosc.2015.09.008

	Environment-induced conformational and functional changes of L-2-haloacid dehalogenase
	Wang, Yayue 1,2; Cao, Xupeng 1; Feng, Yanbin 1; Xue, Song 1
刊名	JOURNAL OF BIOSCIENCE AND BIOENGINEERING
	2016-05-01
卷号	121 期号:5 页码:491-496
关键词	L-2-haloacid Dehalogenase Circular Dichroism Environmental Effects Conformation
ISSN号	1389-1723
DOI	10.1016/j.jbiosc.2015.09.008
文献子类	Article
英文摘要	2-Haloacid dehalogenases have been highly studied due to their potential applications in chemical industries and bioremediation. Although biochemical and structural characterizations of the enzyme have been detailed, no information was available regarding environmental effects on the structure function relationship. Here, circular dichroism spectroscopy (CD) was used to investigate the correlation between changes on the conformation and the function of L-2-haloacid dehalogenase (HadL AJ1) from the Pseudomonas putida induced by the environmental factors. Decreased alpha-helix and increased beta-sheet contents were observed in the structure of HadL AJ1 along with activity losses caused by pH, temperature and inhibitors. Regardless of which factor above-mentioned existed, more than 65.0% of HadL AJ1 activity could be remained if its alpha-helix content was over 12.0%. The maintenance of alpha-helical structure in HadL All was indispensable to its catalysis, while beta-sheet increase restricts its activity. This study revealed the variation of enzymatic activity due to environmental conditions resulting in structural changes monitored by CD, which contributed to rational modification and was instructive for predicting changes of the enzymatic activity during application. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
WOS关键词	BACTERIAL 2-HALOACID DEHALOGENASES ; XANTHOBACTER-AUTOTROPHICUS GJ10 ; SPONGE HYMENIACIDON-PERLEVIS ; BURKHOLDERIA-CEPACIA MBA4 ; PSEUDOMONAS SP. YL ; HALOACID DEHALOGENASE ; CIRCULAR-DICHROISM ; ACID DEHALOGENASE ; CRYSTAL-STRUCTURE ; THERMAL-DENATURATION
WOS研究方向	Biotechnology & Applied Microbiology ; Food Science & Technology
语种	英语
出版者	SOC BIOSCIENCE BIOENGINEERING JAPAN
WOS记录号	WOS:000376695300003
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170992]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xue, Song
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yayue,Cao, Xupeng,Feng, Yanbin,et al. Environment-induced conformational and functional changes of L-2-haloacid dehalogenase[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2016,121(5):491-496.
APA	Wang, Yayue,Cao, Xupeng,Feng, Yanbin,&Xue, Song.(2016).Environment-induced conformational and functional changes of L-2-haloacid dehalogenase.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,121(5),491-496.
MLA	Wang, Yayue,et al."Environment-induced conformational and functional changes of L-2-haloacid dehalogenase".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121.5(2016):491-496.