Nogo-B receptor promotes the chemoresistance of human hepatocellular carcinoma via the ubiquitination of p53 protein

	Nogo-B receptor promotes the chemoresistance of human hepatocellular carcinoma via the ubiquitination of p53 protein
	Wang, Haibo 2; Dong, Chengyong 2; Zhao, Baofeng 3,7,8; Long, Fei 2; Liu, Ying 4,5; Liu, Zhenzhen 2; Li, Song 2; Yang, Xuejun 2; Sun, Deguang 2; Liu, Qinlong 2
刊名	ONCOTARGET
	2016-02-23
卷号	7 期号:8 页码:8850-8865
关键词	Ngbr Chemoresistance Hcc
ISSN号	1949-2553
文献子类	Article
英文摘要	Nogo-B receptor (NgBR), a type I single transmembrane domain receptor is the specific receptor for Nogo-B. Our previous work demonstrated that NgBR is highly expressed in breast cancer cells, where it promotes epithelial mesenchymal transition (EMT), an important step in metastasis. Here, we show that both in vitro and in vivo increased expression of NgBR contributes to the increased chemoresistance of Bel7402/5FU cells, a stable 5-FU (5-Fluorouracil) resistant cell line related Bel7402 cells. NgBR knockdown abrogates S-phase arrest in Bel7402/5FU cells, which correlates with a reduction in G1/S phase checkpoint proteins p53 and p21. In addition, NgBR suppresses p53 protein levels through activation of the PI3K/Akt/MDM2 pathway, which promotes p53 degradation via the ubiquitin proteasome pathway and thus increases the resistance of human hepatocellular cancer cells to 5-FU. Furthermore, we found that NgBR expression is associated with a poor prognosis of human hepatocellular carcinoma (HCC) patients. These results suggest that targeting NgBR in combination with chemotherapeutic drugs, such as 5-FU, could improve the efficacy of current anticancer treatments.
WOS关键词	CELL LUNG-CANCER ; MULTIDRUG-RESISTANCE ; SIGNALING PATHWAY ; MESSENGER-RNA ; CHEMOTHERAPY ; EXPRESSION ; IDENTIFICATION ; STATISTICS ; REGULATOR ; APOPTOSIS
WOS研究方向	Oncology ; Cell Biology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000375618100032
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170914]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Miao, Qing Robert; Wang, Liming
作者单位	1.Chinese Acad Sci, Key Lab Biomed Effects Nanomat & Nanosafety, Natl Ctr Nanosci & Technol China, Beijing, Peoples R China 2.Dalian Med Univ, Div Hepatobiliary & Pancreat Surg, Dept Gen Surg, Affiiated Hosp 2, Dalian, Liaoning, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 4.Dalian Univ, Affiliated Zhongshan Hosp, Dept Oncol, Dalian 116012, Peoples R China 5.Dalian Med Univ, Inst Canc Stem Cell, Dalian, Liaoning, Peoples R China 6.Dalian Med Univ, Key Lab Prote, Dalian, Liaoning, Peoples R China 7.Med Coll Wisconsin, Dept Surg, Childrens Res Inst, Div Pediat Surg, 8700 W Wisconsin Ave, Milwaukee, WI 53226 USA 8.Med Coll Wisconsin, Dept Pathol, Childrens Res Inst, Div Pediat Pathol, Milwaukee, WI 53226 USA 9.Chinese Acad Sci, Inst High Energy Phys, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Haibo,Dong, Chengyong,Zhao, Baofeng,et al. Nogo-B receptor promotes the chemoresistance of human hepatocellular carcinoma via the ubiquitination of p53 protein[J]. ONCOTARGET,2016,7(8):8850-8865.
APA	Wang, Haibo.,Dong, Chengyong.,Zhao, Baofeng.,Long, Fei.,Liu, Ying.,...&Wang, Liming.(2016).Nogo-B receptor promotes the chemoresistance of human hepatocellular carcinoma via the ubiquitination of p53 protein.ONCOTARGET,7(8),8850-8865.
MLA	Wang, Haibo,et al."Nogo-B receptor promotes the chemoresistance of human hepatocellular carcinoma via the ubiquitination of p53 protein".ONCOTARGET 7.8(2016):8850-8865.