Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip

doi:10.1016/j.imlet.2016.03.010

	Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip
	Lu, Xiaoguang 2; Lv, Chang 3; Qu, Yueyang 4; Luo, Yong 4; Lin, Bingcheng 1; Zhan, Libin 5; Song, Yi 2; Fan, Zhiwei 2
刊名	IMMUNOLOGY LETTERS
	2016-05-01
卷号	173 页码:55-60
关键词	Neutrophil Chemotaxis Sepsis Tlr2 Cxcr2 Tlr2-cxcr2 Pathway Microfluidic Chip
ISSN号	0165-2478
DOI	10.1016/j.imlet.2016.03.010
文献子类	Article
英文摘要	This study aimed to design a microfluidic chip to measure neutrophil chemotaxis, which is a convenient assay to assess the severity and prognosis of sepsis, and to study the mechanisms involved in the variation of neutrophil chemotaxis. Neutrophil chemotaxis was investigated in this microfluidic device by measuring the migration speed of neutrophils following the LPS concentration gradient stimulus. Neutrophils of 32 sepsis patients were divided into three groups according to the seriousness of physician-diagnosed sepsis, and 12 healthy individuals served as controls. Statistical significance was set at an alpha value of P<0.05. Neutrophil chemotaxis was significantly decreased following the seriousness of sepsis. By contrast, in septic neutrophils, the expression of TLR2 was significantly increased, whereas the expression of CXCR2 was significantly decreased. Neutrophil chemotaxis in sepsis was significantly reduced as compared to healthy individuals. We speculated that impaired neutrophil chemotaxis in sepsis was probably mediated by the TLR2-CXCR2 pathway. (C) 2016 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.
WOS关键词	STAPHYLOCOCCUS-AUREUS ; PLASMA-CONCENTRATIONS ; ORGAN FAILURE ; SEPTIC SHOCK ; MIGRATION ; DEPLETION ; LIPOPOLYSACCHARIDE ; RECEPTOR ; PROTEIN
WOS研究方向	Immunology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000375737800007
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170890]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Lu, Xiaoguang
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Liaoning, Peoples R China 2.Dalian Univ, Affiliated Zhongshan Hosp, Dept Emergency Med, 6 JieFang Ave, Dalian 116001, Liaoning, Peoples R China 3.Dalian Univ, Dept Med, Dalian 116622, Liaoning, Peoples R China 4.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Liaoning, Peoples R China 5.Nanjing Univ Chinese Med, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Lu, Xiaoguang,Lv, Chang,Qu, Yueyang,et al. Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip[J]. IMMUNOLOGY LETTERS,2016,173:55-60.
APA	Lu, Xiaoguang.,Lv, Chang.,Qu, Yueyang.,Luo, Yong.,Lin, Bingcheng.,...&Fan, Zhiwei.(2016).Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip.IMMUNOLOGY LETTERS,173,55-60.
MLA	Lu, Xiaoguang,et al."Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip".IMMUNOLOGY LETTERS 173(2016):55-60.