A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples

doi:10.1016/j.snb.2016.03.074

	A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples
	Qian, Xing-Kai 1,3; Wang, Ping 3; Xia, Yang-Liu 3; Dou, Tong-Yi 3; Jin, Qiang 3; Wang, Dan-Dan 3; Hao, Da-Cheng 2; Bi, Xiao-Lin 1; Ge, Guang-Bo 3; Yang, Ling 3
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2016-08-01
卷号	231 页码:615-623
关键词	Catechol-o-methyltransferase Fluorescent Probe High-throughput Detection Bioimaging 7 8-dihydroxy-4-methylcoumarin
ISSN号	0925-4005
DOI	10.1016/j.snb.2016.03.074
文献子类	Article
英文摘要	Catechol-O-methyltransferase (COMT), one of the most important phase II drug metabolizing enzymes, plays important roles in the metabolism of endogenous and xenobiotic catechols. In this study, a highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples was discovered and well characterized. Under physiological conditions, COMT selectively catalyzes the conversion of the probe (7,8-dihydroxy-4-methylcoumarin, DHMC) to 7-hydroxy-8methoxy-4-methylcoumarin (HMMC), which brings a strong turn-on fluorescence signal at 520 nm. The probe substrate has been used for monitoring the real activities of COMT in complex biological samples, as well as for rapid screening of potential COMT inhibitors which are useful in the treatment of Parkinson's diseases Furthermore, the probe has been successfully used to monitor endogenous COMT in living cells for the first time, and the results demonstrate that DHMC is cell membrane permeable and low toxic to the cells. All these features of DHMC suggested that this probe holds great promise for COMT-related regulation and inhibition assays in drug discovery, as well as for further investigation on the biological functions of COMT in living cells. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	METHYL TRANSFERASE ; FLUOROMETRIC-DETERMINATION ; CATALYTIC EFFICIENCY ; ASSAY ; INHIBITORS ; DISORDERS ; ESTROGENS ; ERYTHROCYTES ; SUBSTRATE ; DISEASE
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000374330900070
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170888]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Bi, Xiao-Lin; Ge, Guang-Bo; Yang, Ling
作者单位	1.Dalian Med Univ, Inst Canc Stem Cell, Coll Basic Med Sci, Canc Ctr,Dept Biol Sci, Dalian 116044, Peoples R China 2.Dalian Jiaotong Univ, Dalian 116028, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Qian, Xing-Kai,Wang, Ping,Xia, Yang-Liu,et al. A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,231:615-623.
APA	Qian, Xing-Kai.,Wang, Ping.,Xia, Yang-Liu.,Dou, Tong-Yi.,Jin, Qiang.,...&Yang, Ling.(2016).A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples.SENSORS AND ACTUATORS B-CHEMICAL,231,615-623.
MLA	Qian, Xing-Kai,et al."A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples".SENSORS AND ACTUATORS B-CHEMICAL 231(2016):615-623.