Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules | |
Ma, Xiaojun1; Yu, Weiting1; Gao, Meng1,3; Song, Huiyi1,3; Liu, Xiudong2 | |
刊名 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING |
2016-04-01 | |
卷号 | 121期号:4页码:406-412 |
关键词 | Quorum Sensing Entrapped Low Density Cells With Culture Cell Aggregates Microencapsulation Vibrio Harveyi |
ISSN号 | 1389-1723 |
DOI | 10.1016/j.jbiosc.2015.08.010 |
文献子类 | Article |
英文摘要 | Microcapsule entrapped low density cells with culture (ELDCwc), different from free cell culture, conferred stronger stress resistance and improved cell viability of microorganisms. In this paper, the quorum sensing (QS) system of Vibrio harveyi was used to investigate changes when cells were cultured in microcapsules. Cells in ELDCwc group grew into cell aggregates, which facilitated cell-cell communication and led to increased bioluminescence intensity. Moreover, the luxS-AI-2 system, a well-studied QS signal pathway, was detected as both luxS gene and the AI-2 signaling molecule, and the results were analyzed with respect to QS capacity of unit cell. The V. harveyi of ELDCwc also showed higher relative gene expression and stronger quorum sensing capacity when compared with free cells. In conclusion, the confined microcapsule space can promote the cell aggregates formation, reduce cell-cell communication distance and increase local concentration of signal molecule, which are beneficial to bacterial QS. (C) 2015, The Society for Biotechnology, Japan. All rights reserved. |
WOS关键词 | PROBIOTIC BACTERIA ; ALGINATE ; MICROENCAPSULATION ; EXPRESSION ; STRESS ; GENES ; CELLS ; MICROENVIRONMENT ; HETEROGENEITY ; PERMEABILITY |
WOS研究方向 | Biotechnology & Applied Microbiology ; Food Science & Technology |
语种 | 英语 |
出版者 | SOC BIOSCIENCE BIOENGINEERING JAPAN |
WOS记录号 | WOS:000375161900009 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170842] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Yu, Weiting |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Univ, Coll Environm & Chem Engn, Dalian Econ Technol Dev Zone, Dalian 116622, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Xiaojun,Yu, Weiting,Gao, Meng,et al. Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2016,121(4):406-412. |
APA | Ma, Xiaojun,Yu, Weiting,Gao, Meng,Song, Huiyi,&Liu, Xiudong.(2016).Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,121(4),406-412. |
MLA | Ma, Xiaojun,et al."Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121.4(2016):406-412. |
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