Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6

doi:10.1007/s00792-016-0810-5

	Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6
	Bai, Fengwu 1; Wang, Xiaohui 1,2,3; Chi, Naiyu 3; Du, Yuguang 4; Zhao, Yong 2; Yin, Heng 2
刊名	EXTREMOPHILES
	2016-03-01
卷号	20 期号:2 页码:167-176
关键词	Chitin Exochitinase Cold-adapted Salt-tolerant Pseudoalteromonas Sp.
ISSN号	1431-0651
DOI	10.1007/s00792-016-0810-5
文献子类	Article
英文摘要	We have previously reported a non-processive endo-type chitinase, ChiA, from a newly isolated marine psychrophilic bacterium, Pseudoalteromonas sp. DL-6. In this study, a processive exo-type chitinase, ChiC, was cloned from the same bacterium and characterized in detail. ChiC could hydrolyze crystalline chitin into (GlcNAc)(2) as the only observed product. It exhibited high catalytic activity even at low temperatures, e.g. close to 0 degrees C, or in the presence of 5 M NaCl, suggesting that ChiC was a cold-adapted and highly salt-tolerant chitinase. ChiC could also hydrolyze other substrates, including chitosan and Avicel, indicating its broad substrate specificity. Sequence features indicated that ChiC was a multi-domain protein having a deep substrate-binding groove that was regarded as characteristic of processive exo-chitinases. Enzymatic hydrolysis of chitin by ChiC could be remarkably boosted in the presence of ChiA, suggesting the synergy of ChiC and ChiA. This work provided a new evidence to prove that marine psychrophilic bacteria utilized a synergistic enzyme system to degrade recalcitrant chitin.
WOS关键词	MARINE PSYCHROPHILIC BACTERIUM ; SP STRAIN O-7 ; SERRATIA-MARCESCENS ; RECALCITRANT POLYSACCHARIDES ; BIOCHEMICAL-CHARACTERIZATION ; MORITELLA-MARINA ; BINDING DOMAIN ; BETA-CHITIN ; DEGRADATION ; PROCESSIVITY
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
语种	英语
出版者	SPRINGER JAPAN KK
WOS记录号	WOS:000374832600006
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170830]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhao, Yong; Yin, Heng
作者单位	1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Res Grp, Dalian 116023, Peoples R China 3.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Bai, Fengwu,Wang, Xiaohui,Chi, Naiyu,et al. Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6[J]. EXTREMOPHILES,2016,20(2):167-176.
APA	Bai, Fengwu,Wang, Xiaohui,Chi, Naiyu,Du, Yuguang,Zhao, Yong,&Yin, Heng.(2016).Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6.EXTREMOPHILES,20(2),167-176.
MLA	Bai, Fengwu,et al."Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6".EXTREMOPHILES 20.2(2016):167-176.