A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides

doi:10.1016/j.chroma.2016.07.028

	A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides
	Fu, Dongmei 2; Peng, Yahui 1; Zhang, Feifang 1; Yang, Bingcheng 1; Yu, Long 2; Liang, Xinmiao 1,2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2016-08-19
卷号	1460 页码:197-201
关键词	Glycopeptides Hydrophilic Interaction Chromatography Hyperbranched Polyethylenimine Solid Phase Extraction
ISSN号	0021-9673
DOI	10.1016/j.chroma.2016.07.028
文献子类	Article
英文摘要	Selective enrichment and purification of N-glycopeptides from complex biological samples is often a necessary step for effective identification of low abundance glycopeptides. A polyethylenimine functionalized sorbent described here demonstrates good enrichment ability and excellent selectivity towards acidic glycopeptides (e.g. sialylated glycopeptides), even at 1:3000 mass ratio of target glycopeptides and non-glycopeptides, which is much more superior to commercial ZIC-HILIC and other reported sorbents. It also shows effective enrichment for neutral glycopeptides (IgG). Another desirable feature of such sorbent is its large binding capacity (similar to 220 mg/g for fetuin). (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	INTERACTION LIQUID-CHROMATOGRAPHY ; FUNCTIONALIZED STATIONARY-PHASE ; LECTIN AFFINITY-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; EXCHANGE CHROMATOGRAPHY ; EXTRACTION ; GLYCOPROTEINS ; MALTOSE ; SILICA ; MATRIX
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000381536400023
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170040]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yang, Bingcheng
作者单位	1.East China Univ Sci & Technol, Sch Pharm, 130 Meilong RD, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Fu, Dongmei,Peng, Yahui,Zhang, Feifang,et al. A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1460:197-201.
APA	Fu, Dongmei,Peng, Yahui,Zhang, Feifang,Yang, Bingcheng,Yu, Long,&Liang, Xinmiao.(2016).A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides.JOURNAL OF CHROMATOGRAPHY A,1460,197-201.
MLA	Fu, Dongmei,et al."A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides".JOURNAL OF CHROMATOGRAPHY A 1460(2016):197-201.