A crucial role for spatial distribution in bacterial quorum sensing

doi:10.1038/srep34695

	A crucial role for spatial distribution in bacterial quorum sensing
	Lou, Ruyun 1,2; Gao, Meng 1,2; Zheng, Huizhen 1,2; Ren, Ying 1,2; Wu, Fan 1,2; Yu, Weiting 1; Liu, Xiudong 3; Ma, Xiaojun 1
刊名	SCIENTIFIC REPORTS
	2016-10-04
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep34695
文献子类	Article
英文摘要	Quorum sensing (QS) is a process that enables bacteria to communicate using secreted signaling molecules, and then makes a population of bacteria to regulate gene expression collectively and control behavior on a community-wide scale. Theoretical studies of efficiency sensing have suggested that both mass-transfer performance in the local environment and the spatial distribution of cells are key factors affecting QS. Here, an experimental model based on hydrogel microcapsules with a three-dimensional structure was established to investigate the influence of the spatial distribution of cells on bacterial QS. Vibrio harveyi cells formed different spatial distributions in the microcapsules, i.e., they formed cell aggregates with different structures and sizes. The cell aggregates displayed stronger QS than did unaggregated cells even when equal numbers of cells were present. Large aggregates (LA) of cells, with a size of approximately 25 mu m, restricted many more autoinducers (AIs) than did small aggregates (SA), with a size of approximately 10 mu m, thus demonstrating that aggregate size significantly affects QS. These findings provide a powerful demonstration of the fact that the spatial distribution of cells plays a crucial role in bacterial QS.
WOS关键词	ESCHERICHIA-COLI ; VIBRIO-HARVEYI ; CELL MICROENCAPSULATION ; BIOFILM FORMATION ; MICROCAPSULES ; DIFFUSION ; ALGINATE ; COMMUNICATION ; AGGREGATION ; EXPRESSION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000384711300002
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169998]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yu, Weiting; Liu, Xiudong
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ, Coll Environm & Chem Engn, Dalian Econ Technol Dev Zone, Dalian 116622, Peoples R China
推荐引用方式 GB/T 7714	Lou, Ruyun,Gao, Meng,Zheng, Huizhen,et al. A crucial role for spatial distribution in bacterial quorum sensing[J]. SCIENTIFIC REPORTS,2016,6.
APA	Lou, Ruyun.,Gao, Meng.,Zheng, Huizhen.,Ren, Ying.,Wu, Fan.,...&Ma, Xiaojun.(2016).A crucial role for spatial distribution in bacterial quorum sensing.SCIENTIFIC REPORTS,6.
MLA	Lou, Ruyun,et al."A crucial role for spatial distribution in bacterial quorum sensing".SCIENTIFIC REPORTS 6(2016).