Probing the response of lung tumor cells to inflammatory microvascular endothelial cells on fluidic microdevice

doi:10.1002/elps.201600278

	Probing the response of lung tumor cells to inflammatory microvascular endothelial cells on fluidic microdevice
	Guo, Yaqiong 2; Qin, Jianhua 2; Xu, Hui 1,2; Li, Zhongyu 1,2; Peng, Xiaojun 1
刊名	ELECTROPHORESIS
	2017
卷号	38 期号:2 页码:311-319
关键词	Cell Adhesion Cell Rolling Microfluidics Microvascular Inflammation
ISSN号	0173-0835
DOI	10.1002/elps.201600278
文献子类	Article
英文摘要	The development of cancer depends on a complex tissue microenvironment for sustained growth, invasion, and metastasis. The extravasation of tumor cells is a critical event in tumor metastasis. However, the process and mechanism that underlie tumor cell extravasation remain unclear, which restricts the examination of many tumor processes and presents a formidable hurdle to drug development. To explore the initial steps by which lung tumor cells interact with the brain microvascular wall in the course of extravasation, we present a simple, inexpensive, and time-saving microfluidic device to mimic the inflammatory brain microvascular microenvironment and to investigate both the biochemical and mechanical causes of lung tumor cell rolling and adhesion on inflammatory endothelium to analyze the synergistic effects on tumor extravasation under fluidic shear stress conditions. Under microvascular inflammation induced by tumor necrosis factor , the lung tumor cells (A549 cells) displayed significant adhesion activity. In addition, we found that this situation could be reversed by administration of Rho/Rho-associated protein serine/threonine kinase (ROCK) inhibitor (Y27632). We believe that this promising microdevice-based tumor adhesion and extravasation research platform can be used to study tumor behavior in an inflammatory vascular system and will make a valuable contribution for the investigation of the mechanism of tumor cell extravasation.
WOS关键词	SHEAR-STRESS ; BRAIN METASTASIS ; BARRIER FUNCTION ; MIGRATION ; EXPRESSION ; GLIOBLASTOMA ; NEUTROPHILS ; ADHESION ; MODEL ; CHIP
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000393298200010
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169686]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Qin, Jianhua
作者单位	1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Guo, Yaqiong,Qin, Jianhua,Xu, Hui,et al. Probing the response of lung tumor cells to inflammatory microvascular endothelial cells on fluidic microdevice[J]. ELECTROPHORESIS,2017,38(2):311-319.
APA	Guo, Yaqiong,Qin, Jianhua,Xu, Hui,Li, Zhongyu,&Peng, Xiaojun.(2017).Probing the response of lung tumor cells to inflammatory microvascular endothelial cells on fluidic microdevice.ELECTROPHORESIS,38(2),311-319.
MLA	Guo, Yaqiong,et al."Probing the response of lung tumor cells to inflammatory microvascular endothelial cells on fluidic microdevice".ELECTROPHORESIS 38.2(2017):311-319.