5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice

doi:10.3892/mmr.2017.6153

	5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice
	Tang, Haiying 1; Xiang, Dan 2; Wang, Feng 3; Mao, Jingwei 1; Tan, Xiaoyan 1; Wang, Yingde 1
刊名	MOLECULAR MEDICINE REPORTS
	2017-03-01
卷号	15 期号:3 页码:1117-1122
关键词	5-aminosalicylic Acid Silicon Dioxide Nanoparticles Ulcerative Colitis
ISSN号	1791-2997
DOI	10.3892/mmr.2017.6153
文献子类	Article
英文摘要	The targeting of 5-aminosalicylic acid (5-ASA), a first-line therapeutic agent for mild to moderate active ulcerative colitis (UC), to the site of inflammation has remained a challenge and an unmet requirement in the treatment of UC. However, nanoscale carriers for targeted drug delivery are promising for pharmacotherapy, and nanoparticles improve the pharmacokinetics of the loaded therapeutics based on their physical properties. To design and prepare 5-ASA-loaded silicon dioxide nanoparticles (5-ASA-SiO2, NPs), a micro-emulsion method was conducted, and their respective therapeutic effects were validated in a mouse model of UC. Cytotoxicity of 5-ASA-SiO2, NPs was detected in vitro using the Cell Counting Kit-8 method. The therapeutic effect of 5-ASA-SiO2, NPs was assessed based on their disease activity index (DAI), colon histopathology, myeloperoxidase (MPO) and levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). SiO2, NPs were successfully prepared, and cytotoxicity of 5-ASA-SiO2, NPs was identified as being similar to 5-ASA and SiO2, NPs. DAI and colonic histopathology scores in the normal dosage, high dosage and the 5-ASA-SiO2, NP groups demonstrated a significant improvement when compared with the model group. DAI in the high dosage and 5-ASA-SiO2, NP groups also demonstrated a significant improvement when compared with the normal dosage group. However, MPO, serum IL-6 and TNF-a levels in normal dosage, high dosage and 5-ASA-SiO2, NPs groups were significantly lower than in the model group, and these indexes in the high dosage group and 5-ASA-SiO2, NP group were significantly lower than that in the normal dosage group. Expression of IL-6 and TNF-a mRNA in colonic mucosa in the normal dosage, high dosage and 5-ASA-SiO2, NP group was significantly lower than that in the model group. Colonic mucosal IL-6 and TNF-alpha mRNA expression in the high dosage and 5-ASA-SiO2, NP groups was significantly lower than that in the normal dosage group (P<0.05). In conclusion, 5-ASA-SiO2, NPs are a selective drug release system that target the inflamed colon, characteristics of UC, and can greatly increase therapeutic efficacy in UC.
WOS关键词	INFLAMMATORY-BOWEL-DISEASE ; COLONIC MUCOSA ; PREVALENCE ; IBD
WOS研究方向	Oncology ; Research & Experimental Medicine
语种	英语
出版者	SPANDIDOS PUBL LTD
WOS记录号	WOS:000397173000016
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169416]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wang, Yingde
作者单位	1.Dalian Med Univ, Affiliated Hosp 1, Dept Gastroenterol, 222 Zhongshan Rd, Dalian 116011, Liaoning, Peoples R China 2.Sixth Peoples Hosp Shenyang, Dept Liver Dis, Shenyang 110000, Liaoning, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Tang, Haiying,Xiang, Dan,Wang, Feng,et al. 5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice[J]. MOLECULAR MEDICINE REPORTS,2017,15(3):1117-1122.
APA	Tang, Haiying,Xiang, Dan,Wang, Feng,Mao, Jingwei,Tan, Xiaoyan,&Wang, Yingde.(2017).5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice.MOLECULAR MEDICINE REPORTS,15(3),1117-1122.
MLA	Tang, Haiying,et al."5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice".MOLECULAR MEDICINE REPORTS 15.3(2017):1117-1122.