Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs

doi:10.1039/c7tx00209b

	Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs
	Wang, Yaqing 1,2; Su, Wentao 1; Qin, Jianhua 1,2; Jiang, Lei 1; Liu, Yang 1,2; Wang, Li 1; Hui, Lijian 2,3
刊名	TOXICOLOGY RESEARCH
	2018
卷号	7 期号:1 页码:13-21
ISSN号	2045-452X
DOI	10.1039/c7tx00209b
文献子类	Article
英文摘要	Preservation of hepatic phenotype and functions in vitro has always been a great challenge for the reconstruction of liver tissue engineering and in pharmaceutical research studies. Human induced hepatocytes (hiHeps) generated from fibroblasts can be reproducible with almost normal levels of liver specific functions, which are considered as a new source of hepatocytes for biomedical applications. Moreover, paper has served as an attractive biocompatible material for cell-based applications. In this study, we established a simple paper-based scaffold array for creating a 3D liver co-culture model that enabled the assessment of drug induced hepatotoxicity. The hiHeps co-cultured with HUVECs exhibited a 3D like morphology and maintained the liver specific functions of producing albumin and urea for up to 2 months. In addition, the hiHeps in this co-cultured model maintained a higher expression of cytochrome P450 genes as compared with a monolayer culture on a plate and a single culture on paper of hiHeps, revealing a marked enhancement of hepatic functions in the 3D liver co-culture model. Moreover, the 3D liver co-culture model was exposed to acetaminophen (APAP) and pioglitazone, exhibiting near physiological hepatotoxic responses compared to those of the monolayer cultures. Taken together, the low-cost and bioactive paper scaffold could offer great opportunities as 3D in vitro platforms for tissue engineering applications and high-throughput drug testing.
WOS关键词	PRIMARY HUMAN HEPATOCYTES ; IN-VITRO SYSTEMS ; ON-A-CHIP ; ENDOTHELIAL-CELLS ; ENZYME-INDUCTION ; CULTURE ; METABOLISM ; TISSUE ; TRANSPORTER ; 2D
WOS研究方向	Toxicology
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000419189000002
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169176]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Qin, Jianhua
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yaqing,Su, Wentao,Qin, Jianhua,et al. Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs[J]. TOXICOLOGY RESEARCH,2018,7(1):13-21.
APA	Wang, Yaqing.,Su, Wentao.,Qin, Jianhua.,Jiang, Lei.,Liu, Yang.,...&Hui, Lijian.(2018).Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs.TOXICOLOGY RESEARCH,7(1),13-21.
MLA	Wang, Yaqing,et al."Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs".TOXICOLOGY RESEARCH 7.1(2018):13-21.