A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement

doi:10.1016/j.bios.2018.01.034

	A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement
	Li, Tian 1,2; Wu, Xi 2; Tao, Guangyu 2; Yin, Haoyan 3; Zhang, Junlong 3; Liu, Feng 2; Li, Na 2
刊名	BIOSENSORS & BIOELECTRONICS
	2018-05-15
卷号	105 页码:137-142
关键词	Gold Nanoparticle Dark-field Imaging Enumeration Nucleic Acid Target-induced Strand-displacement
ISSN号	0956-5663
DOI	10.1016/j.bios.2018.01.034
文献子类	Article
英文摘要	By combining the gold nanoparticle (AuNP) enumeration with target-induced strand-displacement reaction, we have developed a non-amplification platform for DNA and miRNA detection based on a deliberately designed sandwich-structured nanocomplex probe (SNC Probe). The proposed strategy can realize the sensitive detection of nucleic acids within 40 min with the detection limit of 6.6 fM for HBV DNA and 13.5 fM for miRNA-141, respectively. The method presents reasonable ability to discriminate miRNA-141 from all the other members of miRNA-200 family. And it can also be used for direct detection of miRNA-141 in samples of extracted total small RNA from different cell lines which are reported to have altered levels of miRNA-141. Furthermore, the spike recovery (n = 3) of miRNA-141 in total small RNA extracts of Hela cells is found to be 92.8% for 20 p.M. and 94.7% for 100 p.M. with the standard deviation of 9.2% and 6.8%, respectively. As the only reagent involved in the assay, the SNC Probe presents a very good stability with a relative standard deviation of 3.3% amongst eight tests in 30 days, which greatly simplifies the assay procedure and presents the suitability for routine analyses. On the basis of these findings, this simple non-amplification assay platform can be expected to find assorted applications that can make the best use of the simplicity and sensitivity.
WOS关键词	DARK-FIELD MICROSCOPY ; SINGLE PLASMONIC NANOPARTICLES ; NUCLEIC-ACID DETECTION ; LIGHT-SCATTERING ; LIVING CELLS ; ONE-STEP ; PARTICLE LEVEL ; HYBRIDIZATION ; QUANTIFICATION ; NANOMATERIALS
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000426024200021
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168672]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Li, Na
作者单位	1.Henan Univ Sci & Technol, Med Coll, Luoyang 471003, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,BNLMS, Beijing 100871, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Li, Tian,Wu, Xi,Tao, Guangyu,et al. A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement[J]. BIOSENSORS & BIOELECTRONICS,2018,105:137-142.
APA	Li, Tian.,Wu, Xi.,Tao, Guangyu.,Yin, Haoyan.,Zhang, Junlong.,...&Li, Na.(2018).A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement.BIOSENSORS & BIOELECTRONICS,105,137-142.
MLA	Li, Tian,et al."A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement".BIOSENSORS & BIOELECTRONICS 105(2018):137-142.