Probing impaired neurogenesis in human brain organoids exposed to alcohol | |
Zhu, Yujuan2,3,4; Wang, Li2,4; Yin, Fangchao2,3,4; Yu, Yue2,3,4; Wang, Yaqing2,3,4; Shepard, Matthew J.1,5; Zhuang, Zhengping5; Qin, Jianhua2,3,6 | |
刊名 | INTEGRATIVE BIOLOGY |
2017-12-01 | |
卷号 | 9期号:12页码:968-978 |
ISSN号 | 1757-9694 |
DOI | 10.1039/c7ib00105c |
文献子类 | Article |
英文摘要 | The fetal brain is highly vulnerable to ethanol exposure, which can trigger various long-term neuronal disabilities and cognitive dysfunctions. However, a comprehensive understanding of fetal brain development under ethanol exposure is challenging due to the limitations of animal models. Here, we propose a human induced pluripotent stem cell (hiPSC)-based 3D brain organoid model, and explore the mechanisms underlying neural dysfunctions in prenatal alcohol exposure (PAE) in vitro. Brain organoids were examined to resemble brain organogenesis in vivo at early stages during gestation, with specific features of neuronal differentiation, brain regionalization, and cortical organization. With ethanol exposure, the brain organoids displayed attenuated neurite outgrowth and skewed neural maturation. Transcriptome analysis identified a series of new markedly altered genes and enriched pathways, such as GSX2, RSPO2, and the Hippo signaling pathway. These genes or pathways, to our knowledge, were reported to be involved in ethanol-induced impaired neurogenesis for the first time. Our new findings might facilitate better understanding of the various postnatal neural disorders observed in individuals with PAE. |
WOS关键词 | PLURIPOTENT STEM-CELLS ; HUMAN NEOCORTEX ; IN-VITRO ; ETHANOL ; DISEASE ; CORTEX ; EXPRESSION ; REGULATORS ; RECEPTORS ; NEURONS |
WOS研究方向 | Cell Biology |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000417759500007 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168436] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Qin, Jianhua |
作者单位 | 1.Univ Virginia, Dept Neurol Surg, Charlottesville, VA USA 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Beijing, Peoples R China 5.NINDS, Surg Neurol Branch, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA 6.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Zhu, Yujuan,Wang, Li,Yin, Fangchao,et al. Probing impaired neurogenesis in human brain organoids exposed to alcohol[J]. INTEGRATIVE BIOLOGY,2017,9(12):968-978. |
APA | Zhu, Yujuan.,Wang, Li.,Yin, Fangchao.,Yu, Yue.,Wang, Yaqing.,...&Qin, Jianhua.(2017).Probing impaired neurogenesis in human brain organoids exposed to alcohol.INTEGRATIVE BIOLOGY,9(12),968-978. |
MLA | Zhu, Yujuan,et al."Probing impaired neurogenesis in human brain organoids exposed to alcohol".INTEGRATIVE BIOLOGY 9.12(2017):968-978. |
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