Analysis of normal and modified nucleosides in urine by capillary electrophoresis

	Analysis of normal and modified nucleosides in urine by capillary electrophoresis
	Liebich, HM; Xu, G; DiStefano, C; Lehmann, R; Haring, HU; Lu, P; Zhang, Y
刊名	CHROMATOGRAPHIA
	1997
卷号	45 页码:396-401
关键词	Capillary Electrophoresis Nucleosides And Modified Nucleosides Cancer Urine Tumor Marker
ISSN号	0009-5893
文献子类	Article
英文摘要	Modified nucleosides excreted in urine have been studied as potential diagnostic markers for cancer and AIDS, and as indicators for the whole-body turnover of RNA. Until now reversed-phase (RP) HPLC and, to some extent, immunoassays are the preferred analytical methods for urinary nucleosides. A new capillary electrophoretic method for the analysis of normal and modified nucleosides in urine has been developed and optimized in our laboratory. The separation of nucleosides extracted from normal human urine on phenyl boronic acid affinity chromatography columns was performed in uncoated 565 mm (500 mm to detection window) x 50 mu m i.d. capillary tubing using a 300 mM SDS - 25 mM berate - 50 mM phosphate buffer (pH 6.7), a 45-s load, a voltage of 7.5 kV (41 mu A) and UV detection at 260 and 210 nm. The average recovery of the nucleosides was 91 %. The calibration curves were linear over all physiological and pathophysiological concentration ranges and the limits of detection were at micromolar levels. Reproducibility of migration times were better than 1 % (coefficient of variation, CV), and the reproducibilities of the determined concentrations were better than 5 % for standards and 6-15 % for extracted urine. The developed method was used to quantify 15 normal and modifled nucleosides in 25 normal urines to establish reference ranges. The analysis time was less than 45 min.
WOS关键词	TUMOR-MARKERS ; CANCER-PATIENTS ; MONOCLONAL-ANTIBODIES ; CHROMATOGRAPHY ; ACID ; SEPARATION ; EXCRETION ; TURNOVER ; RNA ; PSEUDOURIDINE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	VIEWEG
WOS记录号	WOS:A1997XA09900063
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168208]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liebich, HM
作者单位	CHINESE ACAD SCI,DALIAN INST CHEM PHYS,NATL CHROMATOGRAPH R&A CTR,DALIAN 116011,PEOPLES R CHINA
推荐引用方式 GB/T 7714	Liebich, HM,Xu, G,DiStefano, C,et al. Analysis of normal and modified nucleosides in urine by capillary electrophoresis[J]. CHROMATOGRAPHIA,1997,45:396-401.
APA	Liebich, HM.,Xu, G.,DiStefano, C.,Lehmann, R.,Haring, HU.,...&Zhang, Y.(1997).Analysis of normal and modified nucleosides in urine by capillary electrophoresis.CHROMATOGRAPHIA,45,396-401.
MLA	Liebich, HM,et al."Analysis of normal and modified nucleosides in urine by capillary electrophoresis".CHROMATOGRAPHIA 45(1997):396-401.