Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells

doi:10.1186/s12885-016-2595-4

	Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells
	Ma,Xiao-jun 1; Liu,Chang 1,2; Liu,Yang 1; Xu,Xiao-xi 1,2; Guo,Xin 1; Sun,Guang-wei 1
刊名	BMC Cancer
	2016-07-30
卷号	16 期号:1
关键词	Three-dimensional cell culture Umbilical cord mensenchymal stem cells Hepatocellular carcinoma Metastasis TGF-β
ISSN号	1471-2407
DOI	10.1186/s12885-016-2595-4
通讯作者	Sun,Guang-wei(sungw@dicp.ac.cn)
英文摘要	AbstractBackgroundAccumulating evidences have demonstrated that mesenchymal stem cells (MSC) could be recruited to the tumor microenvironment. Umbilical cord mesenchymal stem cells (UCMSC) were attractive vehicles for delivering therapeutic agents against cancer. Nevertheless, the safety of UCMSC in the treatment of tumors including hepatocellular carcinoma (HCC) was still undetermined.MethodsIn this study, an in vitro co-culture system was established to evaluate the effect of UCMSC on the cell growth, cancer stem cell (CSC) characteristics, drug resistance, metastasis of 3D-cultured HCC cells, and the underlying mechanism was also investigated.ResultsIt was found that after co-cultured with UCMSC, the metastatic ability of 3D-cultured HCC cells was significantly enhanced as indicated by up-regulation of matrix metalloproteinase (MMP), epithelial-mesenchymal transition (EMT)-related genes, and migration ability. However, cell growth, drug resistance and CSC-related gene expression of HCC cells were not affected by UCMSC. Moreover, EMT was reversed, MMP-2 expression was down-regulated, and migration ability of HCC cell was significantly inhibited when TGF-β receptor inhibitor SB431542 was added into the co-culture system.ConclusionsTherefore, these data indicated that UCMSC could significantly enhance the tumor cell metastasis, which was due to the EMT of HCC cells induced by TGF-β.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S12885-016-2595-4
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/167098]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Sun,Guang-wei
作者单位	1.Laboratory of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences 2.University of Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Ma,Xiao-jun,Liu,Chang,Liu,Yang,et al. Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells[J]. BMC Cancer,2016,16(1).
APA	Ma,Xiao-jun,Liu,Chang,Liu,Yang,Xu,Xiao-xi,Guo,Xin,&Sun,Guang-wei.(2016).Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells.BMC Cancer,16(1).
MLA	Ma,Xiao-jun,et al."Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells".BMC Cancer 16.1(2016).