Highly Specific Enrichment of Multi-phosphopeptides by the Diphosphorylated Fructose-Modified Dual-Metal-Centered Zirconium-Organic Framework

doi:10.1021/acsami.8b11138

	Highly Specific Enrichment of Multi-phosphopeptides by the Diphosphorylated Fructose-Modified Dual-Metal-Centered Zirconium-Organic Framework
	Wu, Ren'an 2; Peng, Jiaxi 2,3; Niu, Huan 2,3; Zhang, Hongyan 2,3; Yao, Yating 3; Zhao, Xingyun 2,3; Zhou, Xiaoyu 2,3; Wan, Lihong 2; Kang, Xiaohui 1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018-09-26
卷号	10 期号:38 页码:32613-32621
关键词	metal-organic framework multi-phosphopeptide diphosphorylated fructose selective capture interaction mechanism
ISSN号	1944-8244
DOI	10.1021/acsami.8b11138
通讯作者	Kang, Xiaohui(kangxh@dmu.edu.cn) ; Wu, Ren'an(wurenan@dicp.ac.cn)
英文摘要	Multisite phosphorylation of a protein, generally occurring in biological processes, plays important roles in the regulation of cellular functions. However, the identification of multi-phosphopeptides especially at low abundance is a big challenge as the extreme hydrophilicity and poor ionization efficiency of the multiphosphorylated peptides restrict the deep inspection of multisite phosphorylation processes. In this study, the highly specific enrichment of multiphosphorylated peptides was achieved via the modification of the dual-metal-centered zirconium-organic framework with the diphosphorylated fructose. The diphosphorylated fructose-modified dual-metal-centered zirconium-organic framework (DZMOF-FDP) demonstrated the highly specific affinity to the multiple phosphorylated peptides, with the density functional theory calculations explaining the plausible mechanism for multi-phosphopeptides on the DZMOF-FDP. The selective capture of multi-phosphopeptides from mimic samples confirmed the superior performance of the DZMOF-FDP, with comprehensive comparisons to other modification agents, such as orthophosphate and pyrophosphate. A number of 1871 multiphosphorylated peptides captured by DZMOF-FDP from tryptic digests of HeLa cell lysate could be identified, significantly higher than that by the pristine DZMOF. The deliberately designed modification with diphosphorylated fructose for the dual-zirconium-centered metal-organic framework materials suggests an efficient strategy to develop new enrichment methods in the selective capture of target analytes by judiciously optimizing specific modifiers for adsorbents.
资助项目	National Natural Science Foundation of China[21675156] ; National Natural Science Foundation of China[21375125] ; National Natural Science Foundation of China[21704011] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201503] ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics[DICP TMSR201601]
WOS关键词	PHOSPHOPROTEOME RESEARCH ; PHOSPHORYLATED PEPTIDES ; SIGNALING NETWORKS ; AFFINITY PROBE ; NANOPARTICLES ; SELECTIVITY ; REMOVAL ; MS
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000446142100088
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/167000]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wu, Ren'an; Kang, Xiaohui
作者单位	1.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Lab High Resolut Mass Spectrometry Technol, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wu, Ren'an,Peng, Jiaxi,Niu, Huan,et al. Highly Specific Enrichment of Multi-phosphopeptides by the Diphosphorylated Fructose-Modified Dual-Metal-Centered Zirconium-Organic Framework[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(38):32613-32621.
APA	Wu, Ren'an.,Peng, Jiaxi.,Niu, Huan.,Zhang, Hongyan.,Yao, Yating.,...&Kang, Xiaohui.(2018).Highly Specific Enrichment of Multi-phosphopeptides by the Diphosphorylated Fructose-Modified Dual-Metal-Centered Zirconium-Organic Framework.ACS APPLIED MATERIALS & INTERFACES,10(38),32613-32621.
MLA	Wu, Ren'an,et al."Highly Specific Enrichment of Multi-phosphopeptides by the Diphosphorylated Fructose-Modified Dual-Metal-Centered Zirconium-Organic Framework".ACS APPLIED MATERIALS & INTERFACES 10.38(2018):32613-32621.