Ultrasensitive Ambient Mass Spectrometry Immunoassays: Multiplexed Detection of Proteins in Serum and on Cell Surfaces

doi:10.1021/jacs.8b10853

	Ultrasensitive Ambient Mass Spectrometry Immunoassays: Multiplexed Detection of Proteins in Serum and on Cell Surfaces
	Xu, Shuting; Bai, Yu; Liu, Huwei; Ma, Wen
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2019-01-09
卷号	141 期号:1 页码:72-75
ISSN号	0002-7863
DOI	10.1021/jacs.8b10853
通讯作者	Bai, Yu(yu.bai@pku.edu.cn)
英文摘要	The sensitive and accurate determination of multiple protein biomarkers for clinical diagnosis is in high demand. Here, an ambient mass spectrometry immunoassay platform that possesses advantages of high sensitivity, multiplexed quantitation, low sample consumption and convenient operation was established. A series of extensible rhodamine-based mass tags that ensured quantification of multiple proteins with ultrahigh sensitivity through two-stage signal amplification were developed. Thrombin was detected at zeptomole sensitivity in 2 mu L serum/plasma, as was free cancer antigen 125 (CA125). Three protein biomarkers (CA125, carcinoembryonic antigen, and epithelial cell adhesion molecule) were simultaneously detected in situ on ca. 20 cells. This platform is promising for multiple protein detection in a single drop of sample or at the single-cell scale for clinical diagnosis and therapy.
资助项目	National Natural Science Foundation of China[21874003] ; National Natural Science Foundation of China[21527809] ; National Natural Science Foundation of China[21575007] ; National Natural Science Foundation of China[21322505] ; Ministry of Science and Technology of the People's Republic of China[2016YFF0100303] ; National Key R&D Program of China[2017YFC0906800]
WOS关键词	CANCER ; EXPRESSION ; BREAST ; MUCINS ; CA125
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000455561800013
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Key R&D Program of China ; National Key R&D Program of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166268]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Bai, Yu
作者单位	Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Xu, Shuting,Bai, Yu,Liu, Huwei,et al. Ultrasensitive Ambient Mass Spectrometry Immunoassays: Multiplexed Detection of Proteins in Serum and on Cell Surfaces[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(1):72-75.
APA	Xu, Shuting,Bai, Yu,Liu, Huwei,&Ma, Wen.(2019).Ultrasensitive Ambient Mass Spectrometry Immunoassays: Multiplexed Detection of Proteins in Serum and on Cell Surfaces.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(1),72-75.
MLA	Xu, Shuting,et al."Ultrasensitive Ambient Mass Spectrometry Immunoassays: Multiplexed Detection of Proteins in Serum and on Cell Surfaces".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.1(2019):72-75.