Rapid Quantitative Fluorescence Detection of Copper Ions with Disposable Microcapsule Arrays Utilizing Functional Nucleic Acid Strategy

doi:10.1038/s41598-018-36842-x

	Rapid Quantitative Fluorescence Detection of Copper Ions with Disposable Microcapsule Arrays Utilizing Functional Nucleic Acid Strategy
	Zhou, Yinglin 3; Zheng, Fengyi 4; Cai, Liangyuan 3; Zhou, Qianyu 3; Guo, Dan 2; Li, Zhihong 1,4; Zhang, Xinxiang 3; He, Enqi 1,2
刊名	SCIENTIFIC REPORTS
	2019-01-10
卷号	9 页码:8
ISSN号	2045-2322
DOI	10.1038/s41598-018-36842-x
通讯作者	Zhou, Yinglin(zhouyl@pku.edu.cn) ; Zhang, Xinxiang(zxx@pku.edu.cn) ; Li, Zhihong(zhhli@pku.edu.cn)
英文摘要	In this work, an economical and easy-to-use microcapsule array fabricated by ice printing technique has been realized for ultrasensitive fluorescence quantification of copper ions employing functional nucleic acid strategy. With ice printing, the detection reagents are sealed by polystyrene (PS) film isolation and photopolymer, which guarantees a stable and contamination-free environment for functional nucleic acid reaction. Our microcapsule arrays have shown long-term stability (20 days) under -20 degrees C storage in frozen form before use. During the Cu2+ on-site detection, 1 mu L sample is simply injected into the thawy microcapsule by a microliter syringe under room temperature, and after 20 minutes the fluorescence result can be obtained by an LED transilluminator. This method can realize the detection limit to 100 nM (100 fmol/mu L) with high specificity.
资助项目	National Key Research and Development Program of China[2016YFA0200802] ; National Natural Science Foundation of China[21675004] ; National Natural Science Foundation of China[21575005] ; National Natural Science Foundation of China[21275009]
WOS关键词	CATALYTIC BEACON SENSOR ; ON-A-CHIP ; HIGH-SENSITIVITY ; DNA ; MERCURY ; ENZYME
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000455352400019
资助机构	National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166226]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhou, Yinglin; Li, Zhihong; Zhang, Xinxiang
作者单位	1.Tsinghua Univ, Ctr Nano & Micro Mech, Beijing 100084, Peoples R China 2.Tsinghua Univ, Dept Mech Engn, State Key Lab Tribol, Beijing 100084, Peoples R China 3.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,BNLMS, Beijing 100871, Peoples R China 4.Peking Univ, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yinglin,Zheng, Fengyi,Cai, Liangyuan,et al. Rapid Quantitative Fluorescence Detection of Copper Ions with Disposable Microcapsule Arrays Utilizing Functional Nucleic Acid Strategy[J]. SCIENTIFIC REPORTS,2019,9:8.
APA	Zhou, Yinglin.,Zheng, Fengyi.,Cai, Liangyuan.,Zhou, Qianyu.,Guo, Dan.,...&He, Enqi.(2019).Rapid Quantitative Fluorescence Detection of Copper Ions with Disposable Microcapsule Arrays Utilizing Functional Nucleic Acid Strategy.SCIENTIFIC REPORTS,9,8.
MLA	Zhou, Yinglin,et al."Rapid Quantitative Fluorescence Detection of Copper Ions with Disposable Microcapsule Arrays Utilizing Functional Nucleic Acid Strategy".SCIENTIFIC REPORTS 9(2019):8.