Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds

doi:10.1186/s13068-019-1470-3

	Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds
	Liu,Qishun 3; Yao,Changhong 1,3; Sun,Yongxin 2; Chen,Wei 3; Tan,Haidong 3; Cao,Xupeng 3; Xue,Song 3; Yin,Heng 3,4
刊名	Biotechnology for Biofuels
	2019-05-24
卷号	12 期号:1
关键词	Arthrospira platensis Carbohydrate Nitrogen limitation Purification
ISSN号	1754-6834
DOI	10.1186/s13068-019-1470-3
通讯作者	Cao,Xupeng(c_x_p@dicp.ac.cn) ; Xue,Song(xuesong@dicp.ac.cn) ; Yin,Heng(yinheng@dicp.ac.cn)
英文摘要	AbstractBackgroundCarbohydrates are major biomass source in fuel-targeted biorefinery. Arthrospira platensis is the largest commercialized microalgae with good environmental tolerance and high biomass production. However, the traditional target of A. platensis cultivation is the protein, which is the downstream product of carbohydrates. Aiming to provide the alternative non-food carbohydrates source, the feasible manipulation technology on the cultivation is needed, as well as new separation methodology to achieve maximum utilization of overall biomass.ResultsThe present study aimed to demonstrate the feasibility of industrially producing carbohydrate-enriched A. platensis and characterize the structure of the polysaccharide involved. Cultivated in industrial-scale outdoor open raceway ponds under nitrogen limitation, A. platensis accumulated maximally 64.3%DW of carbohydrate. The maximum biomass and carbohydrate productivity reached 27.5?g?m?2?day?1 and 26.2?g?m?2?day?1, respectively. The efficient extraction and purification of the polysaccharides include a high-pressure homogenization-assisted hot water extraction followed by flocculation with a non-toxic flocculant ZTC1?+?1, with the polysaccharide purity and total recovery reaching 81% and 75%, respectively. The purified polysaccharide was mainly composed of (1→3)(1→4)- or (1→3)(1→2)-α-glucan with a molecular weight of 300–700?kDa, which differed from the commonly acknowledged glycogen.ConclusionsBy the way of controlled nitrogen limitation, the high carbohydrate production of A. platensis in the industrial scale was achieved. The α-glucan from A. platensis could be a potential glucose source for industrial applications. A non-toxic separation method of carbohydrate was applied to maintain the possibility of utilization of residue in high-value field.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S13068-019-1470-3
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/165688]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Cao,Xupeng; Xue,Song; Yin,Heng
作者单位	1. 2. 3. 4.
推荐引用方式 GB/T 7714	Liu,Qishun,Yao,Changhong,Sun,Yongxin,et al. Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds[J]. Biotechnology for Biofuels,2019,12(1).
APA	Liu,Qishun.,Yao,Changhong.,Sun,Yongxin.,Chen,Wei.,Tan,Haidong.,...&Yin,Heng.(2019).Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds.Biotechnology for Biofuels,12(1).
MLA	Liu,Qishun,et al."Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds".Biotechnology for Biofuels 12.1(2019).