Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization

	Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization
	Ding Yang-Jun ; Zhao Xian-En ; Zhu Fang ; Suo You-Rui ; Li Yu-Lin ; Chen Cui-Chen ; You Jin-Mao
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2007-03-01
英文摘要	A method for the identification of long-chain unsaturated fatty acids in deep-sea fish oil using 1 2-benzo-3, 4-dihydrocarbazole-9-ethyl-benzenesulfonte (BDEBS) as pre-column derivatization reagent by reversed-phase HPLC/MS has been developed. To avoid the migration of double bond in the fatty acid chain, fish oil samples were saponificated and derivatized under nitrogen circumstance. The derivatized solution was extracted with hexane and dried under a stream of nitrogen; the residue was re-dissolved with acetorntrile prior to LC analysis. MS analysis of the fatty acid derivatives exhibited regular information regarding the position of the double bond in the fatty acid chain. With molecular foil mass at m/z [MH](+) and specific fragment ions obtained with MS analysis, the position of the double bond in fatty acid chain was located (or calculated) by means of several established formulas. According to the analysis of HPLC/MS/APCI, 23 fatty acids were identified. The main fatty acids in deep-sea fish oil are C-14-C-22 and 67.08% (area percent) of them are polytinsaturated fatly acids. The main components of the unsaturated fatty acids are C(16:1)9-hexadecenoic acid, 11.7% C16:4 4, 7, 10, 13-hexadecatetraenoic acid, 2.91%; C-18:1 12-octadecenoic acid, 11.1%; C-20:0 6, 9, 12, 15-octadecatetractioic acid, 3.62%; C-20:1 13-eicosenoic acid, 1.21%; C(20:5)5, 8, 11, 14, 17-eicosapentaenoic acid, 16.71%; C(22:6)2, 5, 8, 11, 14, 17-docosahexenoic acid, 10. 53%. The established method provides a new technical tool for the location of doulde bond positions in the, poly unsaturated fatty acids.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6867]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Ding Yang-Jun,Zhao Xian-En,Zhu Fang,et al. Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007.
APA	Ding Yang-Jun.,Zhao Xian-En.,Zhu Fang.,Suo You-Rui.,Li Yu-Lin.,...&You Jin-Mao.(2007).Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Ding Yang-Jun,et al."Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2007).