Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H-brevisubulatum (Poaceae)

	Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H-brevisubulatum (Poaceae)
	Dou, Quanwen ; Liu, Ruijuan ; Yu, Feng
刊名	COMPARATIVE CYTOGENETICS
	2016
英文摘要	Molecular karyotypes of H. bogdanii Wilensky, 1918 (2n = 14), and H. brevisubulatum Link, 1844 ssp. brevisubulatum (2n = 28), were characterized by physical mapping of several repetitive sequences. A total of 18 repeats, including all possible di- or trinucleotide SSR (simple sequence repeat) motifs and satellite DNAs, such as pAs1, 5S rDNA, 45S rDNA, and pSc119.2, were used as probes for fluorescence in situ hybridization on root-tip metaphase chromosomes. Except for the SSR motifs AG, AT and GC, all the repeats we examined produced detectable hybridization signals on chromosomes of both species. A detailed molecular karyotype of the I genome of H. bogdanii is described for the first time, and each repetitive sequence is physically mapped. A high degree of chromosome variation, including aneuploidy and structural changes, was observed in H. brevisubulatum. Although the distribution of repeats in the chromosomes of H. brevisubulatum is different from that of H. bogdanii, similar patterns between the two species imply that the autopolyploid origin of H. brevisubulatum is from a Hordeum species with an I genome. A comparison of the I genome and the other Hordeum genomes, H, Xa and Xu, shows that colocalization of motifs AAC, ACT and CAT and colocalization of motifs AAG and AGG are characteristic of the I genome. In addition, we discuss the evolutionary significance of repeats in the genome during genome differentiation.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6406]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Dou, Quanwen,Liu, Ruijuan,Yu, Feng. Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H-brevisubulatum (Poaceae)[J]. COMPARATIVE CYTOGENETICS,2016.
APA	Dou, Quanwen,Liu, Ruijuan,&Yu, Feng.(2016).Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H-brevisubulatum (Poaceae).COMPARATIVE CYTOGENETICS.
MLA	Dou, Quanwen,et al."Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H-brevisubulatum (Poaceae)".COMPARATIVE CYTOGENETICS (2016).