Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch

	Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch
	Chen, Chen ; Chen, Tao ; Liu, Yongling ; Zou, Denglang ; You, Jinmao ; Li, Yulin
刊名	SEPARATION SCIENCE AND TECHNOLOGY
	2015-03-04
英文摘要	Gentiopicroside, mangiferin, sweroside, and isoorientin, the bioactive constituents of Swertia mussotii Franch, have various pharmacological effects, and are used in particular for treating liver disorders. However, efficient methods for their separation are not currently available. In this study, these bioactive compounds were detected in S. mussotii extracts using high-performance liquid chromatography coupled with mass spectrometry, and separated using a combination of high-speed counter-current chromatography and Sephadex LH-20 column chromatography. Their structures were determined by using H-1 and C-13 nuclear magnetic resonance spectroscopies. The results show that this method is effective for the separation and purification of bioactive compounds from S. mussotii.; Gentiopicroside, mangiferin, sweroside, and isoorientin, the bioactive constituents of Swertia mussotii Franch, have various pharmacological effects, and are used in particular for treating liver disorders. However, efficient methods for their separation are not currently available. In this study, these bioactive compounds were detected in S. mussotii extracts using high-performance liquid chromatography coupled with mass spectrometry, and separated using a combination of high-speed counter-current chromatography and Sephadex LH-20 column chromatography. Their structures were determined by using H-1 and C-13 nuclear magnetic resonance spectroscopies. The results show that this method is effective for the separation and purification of bioactive compounds from S. mussotii.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5573]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Chen,Chen, Tao,Liu, Yongling,et al. Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch[J]. SEPARATION SCIENCE AND TECHNOLOGY,2015.
APA	Chen, Chen,Chen, Tao,Liu, Yongling,Zou, Denglang,You, Jinmao,&Li, Yulin.(2015).Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch.SEPARATION SCIENCE AND TECHNOLOGY.
MLA	Chen, Chen,et al."Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch".SEPARATION SCIENCE AND TECHNOLOGY (2015).