Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling

	Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling
	Li, Guoliang ; Dong, Lihua ; Wang, Aihong ; Wang, Wenli ; Hu, Na ; You, Jinmao
刊名	LWT-FOOD SCIENCE AND TECHNOLOGY ; Li, GL; Dong, LH; Wang, AH; Wang, WL; Hu, N; You, JM.Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling,LWT-FOOD SCIENCE AND TECHNOLOGY,2014,55(1):355
	2014
英文摘要	Biogenic amines (BA) and estrogens (ES) have attracted increasing attentions due to their significance on food safety and food quality. In this study, a novel HPLC-FLD method using ethyl-acridine-sulfonyl chloride (EAC) as fluorescence labeling reagent has been developed for simultaneous determination of BA and ES in food samples. The labeling conditions including labeling time, pH of borate buffers and amount of EAC were optimized. The simultaneous labeling of BA and ES can be finished in as little as 6 min. This method without complex pre-treatment offered the low LOD of 0.27-0.69 ng/mL. It was applied to analyze several food samples (beer, cheese, fish, sausage and shrimp), and showed excellent applicability. (C) 2013 Elsevier Ltd. All rights reserved.; Biogenic amines (BA) and estrogens (ES) have attracted increasing attentions due to their significance on food safety and food quality. In this study, a novel HPLC-FLD method using ethyl-acridine-sulfonyl chloride (EAC) as fluorescence labeling reagent has been developed for simultaneous determination of BA and ES in food samples. The labeling conditions including labeling time, pH of borate buffers and amount of EAC were optimized. The simultaneous labeling of BA and ES can be finished in as little as 6 min. This method without complex pre-treatment offered the low LOD of 0.27-0.69 ng/mL. It was applied to analyze several food samples (beer, cheese, fish, sausage and shrimp), and showed excellent applicability. (C) 2013 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/4294]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Guoliang,Dong, Lihua,Wang, Aihong,et al. Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling[J]. LWT-FOOD SCIENCE AND TECHNOLOGY, Li, GL; Dong, LH; Wang, AH; Wang, WL; Hu, N; You, JM.Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling,LWT-FOOD SCIENCE AND TECHNOLOGY,2014,55(1):355,2014.
APA	Li, Guoliang,Dong, Lihua,Wang, Aihong,Wang, Wenli,Hu, Na,&You, Jinmao.(2014).Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling.LWT-FOOD SCIENCE AND TECHNOLOGY.
MLA	Li, Guoliang,et al."Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling".LWT-FOOD SCIENCE AND TECHNOLOGY (2014).