Isolation, purification and identification of antioxidants from Lepidium latifolium extracts

	Isolation, purification and identification of antioxidants from Lepidium latifolium extracts
	Yuan Xiang ; Wang Haixia ; Mei Lijuan ; Tao Yanduo
刊名	MEDICINAL CHEMISTRY RESEARCH
	2018
英文摘要	Antioxidant potencies of an ethanolic extract and its components from Lepidium latifolium were investigated. In this study, we found that the ethyl acetate soluble fraction of L. latifolium was a rich source of antioxidant, resulting from its high total phenolic content. To determine the antioxidant components of the ethyl acetate fraction, a bioassay-guided fractionation approach using 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate), and ferric reducing antioxidant power assays were conducted. Nine compounds were isolated and their structures were identified by MS and NMR spectral data and comparison to reported data. They are Quercetin-3-O-beta-d-sophoroside-7-O-alpha-l-rhamnoside (1), Apetalumoside B6 (2), Kaempferol-3-O-beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (3), Kaempferol-7-O-alpha-l-rhamnopyranoside (4), Kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-l-rhamnopyranoside (5), Kaempferol-3-O-(2-O-feruloyl-beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranoside)-7-O-glucopyranoside (6), Kaempferol-3-O-beta-D-sophoroside-7-O-alpha-l-rhamnoside (7), Kaempferol-3-O-robinoside-7-O-(2aEuro(3)aEuro(3)-(E)-feruloyl)-sophoroside (8), Quercetin-3-O-(2,6-di-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside-7-O-alpha-l-rhamnopyranoside (9), compounds 1, 2, 4, and 8 had potent free radical scavenging activity. The IC50 values of these compounds were 9.8-12.3 and 7.4-31.4 mu g/mL in DPPH and ABTS assays, respectively. The results indicate that L. latifolium is a potential natural source of antioxidants to treat several diseases related to oxidant by-products of human metabolism.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13299]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yuan Xiang,Wang Haixia,Mei Lijuan,et al. Isolation, purification and identification of antioxidants from Lepidium latifolium extracts[J]. MEDICINAL CHEMISTRY RESEARCH,2018.
APA	Yuan Xiang,Wang Haixia,Mei Lijuan,&Tao Yanduo.(2018).Isolation, purification and identification of antioxidants from Lepidium latifolium extracts.MEDICINAL CHEMISTRY RESEARCH.
MLA	Yuan Xiang,et al."Isolation, purification and identification of antioxidants from Lepidium latifolium extracts".MEDICINAL CHEMISTRY RESEARCH (2018).