Development of ultrasonic-assisted closed in-syringe extraction and derivatization for the determination of labile abietic acid and dehydroabietic acid in cosmetics | |
Liu, Jianjun ; Liu, Mengge ; Li, Xiu ; Lu, Xiaomin ; Chen, Guang ; Sun, Zhiwei ; Li, Guoliang ; Zhao, Xianen ; Zhang, Shijuan ; Song, Cuihua ; Wang, Hua ; Suo, Yourui ; You, Jinmao ; You, JM (reprint author), Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu, Peoples R China. | |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
2014-12-05 | |
英文摘要 | Two resin acids, abietic acid (AA) and dehydroabietic acid (DHAA), in cosmetics may cause allergy or toxicoderma, but remain inaccurately investigated due to their lability. In this work, an accurate, sensitive, efficient and convenient method, utilizing the ultrasonic-assisted closed in-syringe extraction and derivatization (UCSED) prior to high performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD) and on-line tandem mass spectra (MS/MS), has been developed. Analytes are extracted by acetonitrile (10/1, v/m) in a sealed syringe under safe condition (60 degrees C; 15 mm; nitrogen atmosphere) and then in-syringe derivatized by 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-d]imidazol-1-yl) ethyl-p-toluenesulfonate (ANITS) (8-fold, 93 degrees C, 30 min, DMF as co-solvent, K2CO3 as catalyst). In UCSED, derivatization contributes to increase both analytical sensitivity and stability of analytes. Excellent linearity (r(2) >= 0.9991) is achieved in wide range (75-3000 ng/mL (AA); 150-4500 ng/mL (DHAA)). Quite low detection limits (AA: 8.2-10.8 ng/mL; DHAA: 19.4-24.3 ng/mL) and limits of analyte concentration (LOAC) (AA: 30.0-44.5 ng/mL; DHAA: 70.9-86.7 ng/mL) ensure the trace analysis. This method is applied to the analysis of cosmetic samples, including depilatory wax strip, liquid foundation, mascara, eyeliner, eyebrow pencil and lip balm. No additional purification is required and no matrix effect is observed, demonstrating obvious advantages over conventional pretreatment such as solid phase extraction (SPE). Accuracy (RE: -3.2% to 2.51%), precision (RSD: 1.29-2.84%), recovery (95.20-103.63%; 95.51-104.22%) and repeatability (<0.23%; <2.87%) are significantly improved. Furthermore, this work plays a guiding role in developing a reasonable method for labile analytes. (C) 2014 Elsevier B.V. All rights reserved. |
内容类型 | 期刊论文 |
源URL | [http://ir.nwipb.ac.cn/handle/363003/6590] |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Liu, Jianjun,Liu, Mengge,Li, Xiu,et al. Development of ultrasonic-assisted closed in-syringe extraction and derivatization for the determination of labile abietic acid and dehydroabietic acid in cosmetics[J]. JOURNAL OF CHROMATOGRAPHY A,2014. |
APA | Liu, Jianjun.,Liu, Mengge.,Li, Xiu.,Lu, Xiaomin.,Chen, Guang.,...&You, JM .(2014).Development of ultrasonic-assisted closed in-syringe extraction and derivatization for the determination of labile abietic acid and dehydroabietic acid in cosmetics.JOURNAL OF CHROMATOGRAPHY A. |
MLA | Liu, Jianjun,et al."Development of ultrasonic-assisted closed in-syringe extraction and derivatization for the determination of labile abietic acid and dehydroabietic acid in cosmetics".JOURNAL OF CHROMATOGRAPHY A (2014). |
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