Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection | |
Li, Yanxin ; Zhang, Shijuan ; Song, Cuihua ; You, Jinmao ; You, JM (reprint author), Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Peoples R China. | |
刊名 | FOOD ANALYTICAL METHODS
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2013-10-01 | |
英文摘要 | A highly sensitive and selective method was developed for the purification and determination of bisphenol A and alkylphenols in soft drinks by using 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC-Cl) as pre-column labeling reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity of bisphenol A and alkylphenols was greatly enhanced through the introduction of BCEC-Cl moiety with excellent fluorescence property into the target molecules. Meanwhile, the introduction of highly hydrophobic BCEC-Cl moiety into the analytes also greatly increased the hydrophobicity of the target compounds and distinguished them from hydrophilic matrices. Therefore, little interference was observed. Solid-phase extraction with C18 cartridges was applied to sample purification procedure with recoveries of higher than 82 %. When 20 mL of sample was used for analysis, the limits of quantifications of the analytes were between 0.06 and 0.1 mu g L-1. The proposed method was successfully applied to the determination of the target compounds in soft drink samples with a much higher sensitivity than traditional HPLC methods.; A highly sensitive and selective method was developed for the purification and determination of bisphenol A and alkylphenols in soft drinks by using 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC-Cl) as pre-column labeling reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity of bisphenol A and alkylphenols was greatly enhanced through the introduction of BCEC-Cl moiety with excellent fluorescence property into the target molecules. Meanwhile, the introduction of highly hydrophobic BCEC-Cl moiety into the analytes also greatly increased the hydrophobicity of the target compounds and distinguished them from hydrophilic matrices. Therefore, little interference was observed. Solid-phase extraction with C18 cartridges was applied to sample purification procedure with recoveries of higher than 82 %. When 20 mL of sample was used for analysis, the limits of quantifications of the analytes were between 0.06 and 0.1 mu g L-1. The proposed method was successfully applied to the determination of the target compounds in soft drink samples with a much higher sensitivity than traditional HPLC methods. |
内容类型 | 期刊论文 |
源URL | [http://ir.nwipb.ac.cn/handle/363003/3922] ![]() |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Li, Yanxin,Zhang, Shijuan,Song, Cuihua,et al. Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection[J]. FOOD ANALYTICAL METHODS, Li, YX; Zhang, SJ; Song, CH; You, JM.Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection,FOOD ANALYTICAL METHODS,2013,6(5):1284-1290,2013. |
APA | Li, Yanxin,Zhang, Shijuan,Song, Cuihua,You, Jinmao,&You, JM .(2013).Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection.FOOD ANALYTICAL METHODS. |
MLA | Li, Yanxin,et al."Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection".FOOD ANALYTICAL METHODS (2013). |
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