Effects of emodin on the gene expression profiling of human breast carcinoma cells

	Effects of emodin on the gene expression profiling of human breast carcinoma cells
	Huang, Zhiwei ; Chen, Guichen ; Shi, Ping
刊名	CANCER DETECTION AND PREVENTION ; Huang, ZW; Chen, GC; Shi, P.Effects of emodin on the gene expression profiling of human breast carcinoma cells,CANCER DETECTION AND PREVENTION,2009,32(4):286-291
	2009
英文摘要	Background: The mechanism of emodin-mediated cell apoptosis has been investigated extensively in many types of human cancer cells. Our previous study demonstrated that emodin induced apoptosis through the decrease of Bcl-2/Bax ratio and the increase of cytoplasm cytochrome c concentration in human breast cancer BCap-37 cells. However, emodin's reaction to breast cancer cells remains elusive. Materials and methods: An apoptosis-associated cDNA microarray comprised of 458 known genes, namely, death receptors, calpains, death kinases, granzymes, DNA fragmentation proteins, caspases and Bcl-2 family, was used to determine the impact of emodin in breast cancer BCap-37 cells. Furthermore, the candidate emodin target genes were further evaluated via real-time quantitative PCR and Western blot analysis. Results: We found that gene expression profiling in human breast cancer BCap-37 cells was altered when exposed to emodin. Thirty of the unique genes that were either induced or repressed in response to emodin-induced apoptosis were also identified. A follow-up study characterized p53, emodin-induced gene, IGF-2, and emodin-repressed gene, and the downstream proteins were also seen as possible molecular targets of emodin. Conclusion: Data from this study provide novel evidence that emodin induces gene expression profiling changes, but has no effects on caspases. In addition, the p53 pathway may cooperate with the IGF-2 pathway, resulting in an emodin-induced apoptosis through disruption of the mitochondrial signaling pathway in BCap-37 cells. Published by Elsevier Ltd.; Background: The mechanism of emodin-mediated cell apoptosis has been investigated extensively in many types of human cancer cells. Our previous study demonstrated that emodin induced apoptosis through the decrease of Bcl-2/Bax ratio and the increase of cytoplasm cytochrome c concentration in human breast cancer BCap-37 cells. However, emodin's reaction to breast cancer cells remains elusive. Materials and methods: An apoptosis-associated cDNA microarray comprised of 458 known genes, namely, death receptors, calpains, death kinases, granzymes, DNA fragmentation proteins, caspases and Bcl-2 family, was used to determine the impact of emodin in breast cancer BCap-37 cells. Furthermore, the candidate emodin target genes were further evaluated via real-time quantitative PCR and Western blot analysis. Results: We found that gene expression profiling in human breast cancer BCap-37 cells was altered when exposed to emodin. Thirty of the unique genes that were either induced or repressed in response to emodin-induced apoptosis were also identified. A follow-up study characterized p53, emodin-induced gene, IGF-2, and emodin-repressed gene, and the downstream proteins were also seen as possible molecular targets of emodin. Conclusion: Data from this study provide novel evidence that emodin induces gene expression profiling changes, but has no effects on caspases. In addition, the p53 pathway may cooperate with the IGF-2 pathway, resulting in an emodin-induced apoptosis through disruption of the mitochondrial signaling pathway in BCap-37 cells. Published by Elsevier Ltd.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn//handle/363003/1918]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Huang, Zhiwei,Chen, Guichen,Shi, Ping. Effects of emodin on the gene expression profiling of human breast carcinoma cells[J]. CANCER DETECTION AND PREVENTION, Huang, ZW; Chen, GC; Shi, P.Effects of emodin on the gene expression profiling of human breast carcinoma cells,CANCER DETECTION AND PREVENTION,2009,32(4):286-291,2009.
APA	Huang, Zhiwei,Chen, Guichen,&Shi, Ping.(2009).Effects of emodin on the gene expression profiling of human breast carcinoma cells.CANCER DETECTION AND PREVENTION.
MLA	Huang, Zhiwei,et al."Effects of emodin on the gene expression profiling of human breast carcinoma cells".CANCER DETECTION AND PREVENTION (2009).