Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography

	Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography
	Chi, Xiaofeng ; Zhang, Guoying ; Xiao, Yuancan ; Dong, Qi ; Hu, Fengzu
刊名	ASIAN JOURNAL OF CHEMISTRY ; Chi, XF; Zhang, GY; Xiao, YC; Dong, Q; Hu, FZ.Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography,ASIAN JOURNAL OF CHEMISTRY,2014,26(7):1905
	2014-04-01
英文摘要	Dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-diode array detection has been successfully developed for the extraction and determination of sulfonamides in honey samples. The effects of parameters such as the, nature and volume of extraction solvent and dispersive solvent, extraction time, ionic strength and solution pH were investigated. In this 1 method, 1 nit of acetonitrile (as dispersive solvent) containing 40-mu L of 1,1,2,2-tetrachloroethane (as extraction solution) was rapidly injected into a 5 mL honey sample containing the analytes; thereby forming a cloudy solution. After extraction, phase separation was pertained by centrifugation and the enriched analytes in the sedimented,phase Were determined by HPLC-DAD. Under the optimal conditions, the detection limit of the method was 0.8-1.7 mu g kg(-1) and the relative standard deviations (RSD %) for determination of the sulfonamides were in the range of 0.172-1.549 %. Linearity was found to be in the range of 0.1-10.0 mu g mL(-1), also, the enrichment-factors, were in the range of 42-81. Finally, the method was applied to determine the trace amounts of the sulfonamides in real honey sample and satisfactory results Were obtained.; Dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-diode array detection has been successfully developed for the extraction and determination of sulfonamides in honey samples. The effects of parameters such as the, nature and volume of extraction solvent and dispersive solvent, extraction time, ionic strength and solution pH were investigated. In this 1 method, 1 nit of acetonitrile (as dispersive solvent) containing 40-mu L of 1,1,2,2-tetrachloroethane (as extraction solution) was rapidly injected into a 5 mL honey sample containing the analytes; thereby forming a cloudy solution. After extraction, phase separation was pertained by centrifugation and the enriched analytes in the sedimented,phase Were determined by HPLC-DAD. Under the optimal conditions, the detection limit of the method was 0.8-1.7 mu g kg(-1) and the relative standard deviations (RSD %) for determination of the sulfonamides were in the range of 0.172-1.549 %. Linearity was found to be in the range of 0.1-10.0 mu g mL(-1), also, the enrichment-factors, were in the range of 42-81. Finally, the method was applied to determine the trace amounts of the sulfonamides in real honey sample and satisfactory results Were obtained.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/4240]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chi, Xiaofeng,Zhang, Guoying,Xiao, Yuancan,et al. Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography[J]. ASIAN JOURNAL OF CHEMISTRY, Chi, XF; Zhang, GY; Xiao, YC; Dong, Q; Hu, FZ.Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography,ASIAN JOURNAL OF CHEMISTRY,2014,26(7):1905,2014.
APA	Chi, Xiaofeng,Zhang, Guoying,Xiao, Yuancan,Dong, Qi,&Hu, Fengzu.(2014).Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography.ASIAN JOURNAL OF CHEMISTRY.
MLA	Chi, Xiaofeng,et al."Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography".ASIAN JOURNAL OF CHEMISTRY (2014).