An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)

	An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)
	Hu, Yanping ; Xie, Xiaolong ; Wang, Li ; Yang, Jian ; Zhang, Huaigang ; Li, Yi
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY ; Hu, YP; Xie, XL; Wang, L; Yang, J; Zhang, HG; Li, Y.An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae),AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2691-2694
	2009-06-17
英文摘要	Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A(260)/A(280) absorbance ratio of extracted DNA, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. The DNA preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (ISSR). This method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.; Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A(260)/A(280) absorbance ratio of extracted DNA, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. The DNA preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (ISSR). This method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn//handle/363003/1856]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, Yanping,Xie, Xiaolong,Wang, Li,et al. An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, Hu, YP; Xie, XL; Wang, L; Yang, J; Zhang, HG; Li, Y.An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae),AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2691-2694,2009.
APA	Hu, Yanping,Xie, Xiaolong,Wang, Li,Yang, Jian,Zhang, Huaigang,&Li, Yi.(2009).An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae).AFRICAN JOURNAL OF BIOTECHNOLOGY.
MLA	Hu, Yanping,et al."An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)".AFRICAN JOURNAL OF BIOTECHNOLOGY (2009).