Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines

	Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines
	You, Jinmao ; Ding, Chenxu ; Zhu, Fang ; Sun, Xuejun ; Li, Yulin ; Suo, Yourui
刊名	CHROMATOGRAPHIA ; You Jinmao, Ding Chenxu,Zhu Fang,Sun Xuejun,Li Yulin, Suo Yourui. Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines. CHROMATOGRAPHIA,2007,65(9-10): 545-553
	2007-05-01
英文摘要	A highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of Lomatogonium rotatum plants (Tibetan herbal medicine) obtained by solid-phase extraction. The number of hydroxyl groups on the dansylated phenols was estimated by LC-MS-MS analysis in positive-ion mode. Dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic HPLC mobile phases were used. MS fragmentation of the derivatives generated intense protonated molecular ions of m/z [MH](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). Collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. Selected reaction monitoring based on the m/z [MH](+) to 234 and 171 transitions was highly specific for these phenolic compounds. Characteristic ions with m/z values of [MH - 234](+), [MH 2 x 234](+), and [MH - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone.; A highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of Lomatogonium rotatum plants (Tibetan herbal medicine) obtained by solid-phase extraction. The number of hydroxyl groups on the dansylated phenols was estimated by LC-MS-MS analysis in positive-ion mode. Dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic HPLC mobile phases were used. MS fragmentation of the derivatives generated intense protonated molecular ions of m/z [MH](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). Collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. Selected reaction monitoring based on the m/z [MH](+) to 234 and 171 transitions was highly specific for these phenolic compounds. Characteristic ions with m/z values of [MH - 234](+), [MH 2 x 234](+), and [MH - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1276]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	You, Jinmao,Ding, Chenxu,Zhu, Fang,et al. Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines[J]. CHROMATOGRAPHIA, You Jinmao, Ding Chenxu,Zhu Fang,Sun Xuejun,Li Yulin, Suo Yourui. Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines. CHROMATOGRAPHIA,2007,65(9-10): 545-553,2007.
APA	You, Jinmao,Ding, Chenxu,Zhu, Fang,Sun, Xuejun,Li, Yulin,&Suo, Yourui.(2007).Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines.CHROMATOGRAPHIA.
MLA	You, Jinmao,et al."Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines".CHROMATOGRAPHIA (2007).