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	The involvement of suppressors of cytokine signaling 2 (socs2) in immune defense responses of chinese mitten crab eriocheir sinensis
	Zhang, Ying1,2; Zhao, Jianmin1; Zhang, Huan1,2; Gai, Yunchao1,2; Wang, Lingling1; Li, Fengmei1; Yang, Jialong1,2; Qiu, Limei1; Song, Linsheng1
刊名	Developmental and comparative immunology
	2010
卷号	34期号:1页码:42-48
关键词	Eriocheir sinensis Socs2 Listonella anguillarum Micrococcus luteus Immune defense responses
ISSN号	0145-305X
DOI	10.1016/j.dci.2009.08.001
通讯作者	Song, linsheng(lshsong@ms.qdio.ac.cn)
英文摘要	The suppressors of cytokine signaling 2 (socs2) has been identified as negative feedback inhibitors for various cytokines signaling via the jak/stat pathway. in the present studies, the cdna of eriocheir sinensis socs2 (designated as essocs2) was cloned by expressed sequence tag (est) and rapid amplification of cdna ends (race) approaches. the full-length cdna of essocs2 was of 2535 bp, consisting of an open reading frame (a orf) of 1071 bp encoding a polypeptide of 357 amino acids. the deduced amino acid sequence of essocs2 shared 55-62% similarity with other socs2 family members. there were three typical conserved socs family domains in essocs2, including an n-terminal ess formed from a single amphipathic helix, a central sh2 domain with a classic phosphotyrosine (py) site and a c-terminal socs box. the sequence and structural similarity of essocs2 with socs2 proteins from other organisms indicated that essocs2 should be a new member of the socs2 family. phylogenetic analysis revealed that essocs2 was clustered with socs2 from the other invertebrates, and fell into the group of type 11 socs subfamily as a sister branch to cis and socs2 from vertebrate, suggesting the great divergence of socs2 of vertebrate from invertebrate and complex evolution of socs2 family members. the mrna transcript of essocs2 could be detected by semi-quantitative rt-pcr in all examined tissues of healthy crabs, including haemocytes, hepatopancreas, gill, muscle, heart and gonad. the mrna expression of essocs2 in haemocytes was up-regulated to 3.5-fold at 8 h after listonella anguillarum challenge, 3-fold and 3.5-fold at 4 and 6 h, respectively, after micrococcus luteus challenge. these results collectively suggested that essocs2 could be induced by bacteria challenge, and it was involved in the immune defense responses in e. sinensis. (c) 2009 elsevier ltd. all rights reserved.
WOS关键词	SWISS-MODEL ; NEGATIVE REGULATION ; PROTEIN ; IDENTIFICATION ; EXPRESSION ; MICE ; ENVIRONMENT ; (SOCS)-1 ; PATHWAYS ; INNATE
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000272088500005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2414636
专题	中国科学院大学
通讯作者	Song, Linsheng
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ying,Zhao, Jianmin,Zhang, Huan,et al. The involvement of suppressors of cytokine signaling 2 (socs2) in immune defense responses of chinese mitten crab eriocheir sinensis[J]. Developmental and comparative immunology,2010,34(1):42-48.
APA	Zhang, Ying.,Zhao, Jianmin.,Zhang, Huan.,Gai, Yunchao.,Wang, Lingling.,...&Song, Linsheng.(2010).The involvement of suppressors of cytokine signaling 2 (socs2) in immune defense responses of chinese mitten crab eriocheir sinensis.Developmental and comparative immunology,34(1),42-48.
MLA	Zhang, Ying,et al."The involvement of suppressors of cytokine signaling 2 (socs2) in immune defense responses of chinese mitten crab eriocheir sinensis".Developmental and comparative immunology 34.1(2010):42-48.

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