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	Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana
	Ying, Xiao-Bao1,2,3; Dong, Li1,2; Zhu, Hui1,2,3; Duan, Cheng-Guo1,2; Du, Quan-Sheng1,2; Lv, Dian-Qiu1,2,4; Fang, Yuan-Yuan1,2; Antonio Garcia, Juan5; Fang, Rong-Xiang1,2; Guo, Hui-Shan1,2
刊名	Plant cell
	2010-04-01
卷号	22期号:4页码:1358-1372
ISSN号	1040-4651
DOI	10.1105/tpc.109.072058
通讯作者	Guo, hui-shan(guohs@im.ac.cn)
英文摘要	Endogenous eukaryotic rna-dependent rna polymerases (rdrs) produce double-stranded rna intermediates in diverse processes of small rna synthesis in rna silencing pathways. rdr6 is required in plants for posttranscriptional gene silencing induced by sense transgenes (s-ptgs) and has an important role in amplification of antiviral silencing. whereas rdr1 is also involved in antiviral defense in plants, this does not necessarily proceed through triggering silencing. in this study, we show that nicotiana benthamiana transformed with rdr1 from nicotiana tabacum (nt-rdr1 plants) exhibits hypersusceptibility to plum pox potyvirus and other viruses, resembling rdr6-silenced (rdr6i) n. benthamiana. analysis of transient induction of rna silencing in n. benthamiana nt-rdr1 and rdr6i plants revealed that nt-rdr1 possesses silencing suppression activity. we found that nt-rdr1 does not interfere with rdr6-dependent sirna accumulation but turns out to suppress rdr6-dependent s-ptgs. our results, together with previously published data, suggest that rdr1 might have a dual role, contributing, on one hand, to salicylic acid-mediated antiviral defense, and suppressing, on the other hand, the rdr6-mediated antiviral rna silencing. we propose a scenario in which the natural loss-of-function variant of rdr1 in n. benthamiana may be the outcome of selective pressure to maintain a high rdr6-dependent antiviral defense, which would be required to face the hypersensitivity of this plant to a large number of viruses.
WOS关键词	SMALL INTERFERING RNAS ; TOBACCO-MOSAIC-VIRUS ; DIRECTED DNA METHYLATION ; TRANS-ACTING SIRNAS ; SALICYLIC-ACID ; C-ELEGANS ; CAENORHABDITIS-ELEGANS ; ARABIDOPSIS-THALIANA ; ANTIVIRAL DEFENSE ; HELICASE DOMAIN
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000278414900029
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2413538
专题	中国科学院大学
通讯作者	Guo, Hui-Shan
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Heilongjiang Acad Agr Sci, Virus Free Seedling Inst, Haerbin 150086, Heilongjiang, Peoples R China 5.CSIC, Ctr Natl Biotecnol, Dept Plant Mol Genet, E-28049 Madrid, Spain
推荐引用方式 GB/T 7714	Ying, Xiao-Bao,Dong, Li,Zhu, Hui,et al. Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana[J]. Plant cell,2010,22(4):1358-1372.
APA	Ying, Xiao-Bao.,Dong, Li.,Zhu, Hui.,Duan, Cheng-Guo.,Du, Quan-Sheng.,...&Guo, Hui-Shan.(2010).Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana.Plant cell,22(4),1358-1372.
MLA	Ying, Xiao-Bao,et al."Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana".Plant cell 22.4(2010):1358-1372.

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